Human EAAT1 ELISA Kit

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  • Alternative name

    Excitatory amino acid transporter 1 ELISA KIT; Sodium-dependent glutamate/aspartate transporter 1 ELISA KIT; GLAST-1 ELISA KIT; Solute carrier family 1 member 3SLC1A3 ELISA KIT; EAAT1 ELISA KIT; GLAST ELISA KIT; GLAST1 ELISA KIT; GLAST-1 ELISA KIT

  • Catalog
    E013808
  • species
    Human
  • GeneEAAT1
  • Standard CurveHuman EAAT1 ELISA Kit
  • Other Species Mouse EAAT1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EAAT1. No significant cross-reactivity or interference between EAAT1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human EAAT1 is typically less than 0.123ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
  • Intended UseHuman EAAT1 ELISA Kit allows for the in vitro quantitative determination of EAAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EAAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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