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Alternative name
Human 4E-BP1 ELISA Kit;Human eIF4E-binding protein 1 ELISA Kit;Human phosphorylated heat- and acid-stable protein regulated by insulin 1 ELISA Kit;Human PHAS-I ELISA Kit;Human 4EBP1 ELISA Kit;Human BP-1 ELISA Kit;Human eukaryotic translation initiation factor 4E binding protein 1 ELISA Kit;Human eukaryotic translation initiation factor 4E-binding protein 1 ELISA Kit;
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Catalog
E013771
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species
Human
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GeneEIF4EBP1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human EIF4EBP1. No significant cross-reactivity or interference between human EIF4EBP1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human EIF4EBP1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined th
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Intended UseHuman EIF4EBP1 ELISA Kit allows for the in vitro quantitative determination of EIF4EBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF4EBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF4EBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF4EBP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF4EBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human Eukaryotic translation initiation factor 4E-binding protein 1 Protein information
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Uniprot ID
4EBP1_HUMAN
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Uniprot AC
Q13541; B2R502; D3DSW8; Q6IBN3;
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UniGene
Hs.411641;
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GeneID
1978
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KEGG
hsa:1978;
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Human Eukaryotic translation initiation factor 4E-binding protein 1 Protein SEQUENCE
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SEQUENCE 118 AA; 12580 MW; 1682A6BA74132966 CRC64;
MSGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT
RIIYDRKFLM
ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN
SPEDKRAGGE ESQFEMDI
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UCSC
uc003xks.4; human.;
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