SensitivityThe minimum detectable dose of human EIF4EBP1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined th
Intended UseHuman EIF4EBP1 ELISA Kit allows for the in vitro quantitative determination of EIF4EBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF4EBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF4EBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF4EBP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF4EBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Eukaryotic translation initiation factor 4E-binding protein 1 Protein information
Q13541; B2R502; D3DSW8; Q6IBN3;
Human Eukaryotic translation initiation factor 4E-binding protein 1 Protein SEQUENCE