Human EIF4B ELISA Kit

Alternative name
Human EIF-4B ELISA Kit;Human PRO1843 ELISA Kit;Human eukaryotic translation initiation factor 4B ELISA Kit;
Catalog
E013769
species
Human
GeneEIF4B
SpecificityThis assay has high sensitivity and excellent specificity for detection of human EIF4B. No significant cross-reactivity or interference between human EIF4B and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity6.25 pg/ml.
Intended UseHuman EIF4B ELISA Kit allows for the in vitro quantitative determination of EIF4B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF4B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF4B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Eukaryotic translation initiation factor 4B Protein information
Uniprot ID IF4B_HUMAN
Uniprot AC P23588; B4DS13; Q4G0E3; Q53HQ2; Q6GPH5; Q6IB46; Q8WYK5;
UniGene Hs.648394; Hs.702041;
GeneID 1975
KEGG hsa:1975;
Human Eukaryotic translation initiation factor 4B Protein SEQUENCE
SEQUENCE 611 AA; 69151 MW; 31CEFEA865FB10D2 CRC64;
MAASAKKKNK KGKTISLTDF LAEDGGTGGG STYVSKPVSW ADETDDLEGD
VSTTWHSNDD DVYRAPPIDR SILPTAPRAA REPNIDRSRL PKSPPYTAFL
GNLPYDVTEE SIKEFFRGLN ISAVRLPREP SNPERLKGFG YAEFEDLDSL
LSALSLNEES LGNRRIRVDV ADQAQDKDRD DRSFGRDRNR DSDKTDTDWR
ARPATDSFDD YPPRRGDDSF GDKYRDRYDS DRYRDGYRDG YRDGPRRDMD
RYGGRDRYDD RGSRDYDRGY DSRIGSGRRA FGSGYRRDDD YRGGGDRYED
RYDRRDDRSW SSRDDYSRDD YRRDDRGPPQ RPKLNLKPRS TPKEDDSSAS
TSQSTRAASI FGGAKPVDTA AREREVEERL QKEQEKLQRQ LDEPKLERRP
RERHPSWRSE ETQERERSRT GSESSQTGTS TTSSRNARRR ESEKSLENET
LNKEEDCHSP TSKPPKPDQP LKVMPAPPPK ENAWVKRSSN PPARSQSSDT
EQQSPTSGGG KVAPAQPSEE GPGRKDENKV DGMNAPKGQT GNSSRGPGDG
GNRDHWKESD RKDGKKDQDS RSAPEPKKPE ENPASKFSSA SKYAALSVDG
EDENEGEDYA E
UCSC uc001sbh.5; human. [P23588-1];

Category

Human EIF4B ELISA Kit

Human EIF-4B ELISA Kit;Human PRO1843 ELISA Kit;Human eukaryotic translation initiation factor 4B ELISA Kit;