Human EIF4A2 ELISA Kit

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  • Alternative name

    Human eIF-4A-II ELISA Kit;Human eIF4A-II ELISA Kit;Human ATP-dependent RNA helicase eIF4A-2 ELISA Kit;Human DDX2B ELISA Kit;Human EIF4F ELISA Kit;Human BM-010 ELISA Kit;Human EIF4A ELISA Kit;Human eukaryotic translation initiation factor 4A2 ELISA Kit;Human eukaryotic initiation factor 4A-II ELISA Kit;

  • Catalog
    E013768
  • species
    Human
  • GeneEIF4A2
  • Standard CurveHuman EIF4A2 ELISA Kit
  • Other Species Mouse Eif4a2 ELISA KitBovine EIF4A2 ELISA KitChicken EIF4A2 ELISA KitMonkey EIF4A2 ELISA KitRat Eif4a2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman EIF4A2 ELISA Kit allows for the in vitro quantitative determination of EIF4A2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 4A2 (EIF4A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Eukaryotic initiation factor 4A-II Protein information
  • Uniprot ID IF4A2_HUMAN
  • Uniprot AC Q14240; D3DNU9; Q53XJ6; Q96B90; Q96EA8;
  • UniGene Hs.518475; Hs.599481;
  • GeneID 1974
  • KEGG hsa:1974;
  • Human Eukaryotic initiation factor 4A-II Protein SEQUENCE
  • SEQUENCE 407 AA; 46402 MW; FAA7D3BA9D8C6DA0 CRC64;

    MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA

    YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK

    ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA

    EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI

    FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF

    YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV

    SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL

    PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP

    MNVADLI

  • UCSC uc003fqs.4; human. [Q14240-1];


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