Human EIF4A2 ELISA Kit

Alternative name
Human eIF-4A-II ELISA Kit;Human eIF4A-II ELISA Kit;Human ATP-dependent RNA helicase eIF4A-2 ELISA Kit;Human DDX2B ELISA Kit;Human EIF4F ELISA Kit;Human BM-010 ELISA Kit;Human EIF4A ELISA Kit;Human eukaryotic translation initiation factor 4A2 ELISA Kit;Human eukaryotic initiation factor 4A-II ELISA Kit;
Catalog
E013768
species
Human
GeneEIF4A2
Standard Curve
Other Species Mouse Eif4a2 ELISA Kit Bovine EIF4A2 ELISA Kit Chicken EIF4A2 ELISA Kit Monkey EIF4A2 ELISA Kit Rat Eif4a2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseHuman EIF4A2 ELISA Kit allows for the in vitro quantitative determination of EIF4A2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 4A2 (EIF4A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 4A2 (EIF4A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Eukaryotic initiation factor 4A-II Protein information
Uniprot ID IF4A2_HUMAN
Uniprot AC Q14240; D3DNU9; Q53XJ6; Q96B90; Q96EA8;
UniGene Hs.518475; Hs.599481;
GeneID 1974
KEGG hsa:1974;
Human Eukaryotic initiation factor 4A-II Protein SEQUENCE
SEQUENCE 407 AA; 46402 MW; FAA7D3BA9D8C6DA0 CRC64;
MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA
YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK
ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA
EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI
FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF
YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV
SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL
PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP
MNVADLI
UCSC uc003fqs.4; human. [Q14240-1];

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Human EIF4A2 ELISA Kit