Human EIF2AK3 ELISA Kit

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  • Alternative name

    Human PRKR-like endoplasmic reticulum kinase ELISA Kit;Human pancreatic EIF2-alpha kinase ELISA Kit;Human HsPEK ELISA Kit;Human PEK ELISA Kit;Human PERK ELISA Kit;Human WRS ELISA Kit;Human eukaryotic translation initiation factor 2 alpha kinase 3 ELISA Kit;Human eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit;

  • Catalog
    E013734
  • species
    Human
  • GeneEIF2AK3
  • Other Species Mouse Eif2ak3 ELISA KitRat Eif2ak3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman EIF2AK3 ELISA Kit allows for the in vitro quantitative determination of EIF2AK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EIF2AK3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EIF2AK3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EIF2AK3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EIF2AK3 can be calculated.
  • Human Eukaryotic translation initiation factor 2-alpha kinase 3 Protein information
  • Uniprot ID E2AK3_HUMAN
  • Uniprot AC Q9NZJ5; A0AVH1; A0AVH2; B2RCU9; O95846; Q53QY0; Q53SB1;
  • UniGene Hs.591589;
  • GeneID 9451
  • KEGG hsa:9451;
  • Human Eukaryotic translation initiation factor 2-alpha kinase 3 Protein SEQUENCE
  • SEQUENCE 1116 AA; 125216 MW; 95FEB5DAE8D3D452 CRC64;

    MERAISPGLL VRALLLLLLL LGLAARTVAA GRARGLPAPT AEAAFGLGAA

    AAPTSATRVP AAGAVAAAEV TVEDAEALPA AAGEQEPRGP EPDDETELRP

    RGRSLVIIST LDGRIAALDP ENHGKKQWDL DVGSGSLVSS SLSKPEVFGN

    KMIIPSLDGA LFQWDQDRES METVPFTVES LLESSYKFGD DVVLVGGKSL

    TTYGLSAYSG KVRYICSALG CRQWDSDEME QEEDILLLQR TQKTVRAVGP

    RSGNEKWNFS VGHFELRYIP DMETRAGFIE STFKPNENTE ESKIISDVEE

    QEAAIMDIVI KVSVADWKVM AFSKKGGHLE WEYQFCTPIA SAWLLKDGKV

    IPISLFDDTS YTSNDDVLED EEDIVEAARG ATENSVYLGM YRGQLYLQSS

    VRISEKFPSS PKALESVTNE NAIIPLPTIK WKPLIHSPSR TPVLVGSDEF

    DKCLSNDKFS HEEYSNGALS ILQYPYDNGY YLPYYKRERN KRSTQITVRF

    LDNPHYNKNI RKKDPVLLLH WWKEIVATIL FCIIATTFIV RRLFHPHPHR

    QRKESETQCQ TENKYDSVSG EANDSSWNDI KNSGYISRYL TDFEPIQCLG

    RGGFGVVFEA KNKVDDCNYA IKRIRLPNRE LAREKVMREV KALAKLEHPG

    IVRYFNAWLE APPEKWQEKM DEIWLKDEST DWPLSSPSPM DAPSVKIRRM

    DPFATKEHIE IIAPSPQRSR SFSVGISCDQ TSSSESQFSP LEFSGMDHED

    ISESVDAAYN LQDSCLTDCD VEDGTMDGND EGHSFELCPS EASPYVRSRE

    RTSSSIVFED SGCDNASSKE EPKTNRLHIG NHCANKLTAF KPTSSKSSSE

    ATLSISPPRP TTLSLDLTKN TTEKLQPSSP KVYLYIQMQL CRKENLKDWM

    NGRCTIEERE RSVCLHIFLQ IAEAVEFLHS KGLMHRDLKP SNIFFTMDDV

    VKVGDFGLVT AMDQDEEEQT VLTPMPAYAR HTGQVGTKLY MSPEQIHGNS

    YSHKVDIFSL GLILFELLYP FSTQMERVRT LTDVRNLKFP PLFTQKYPCE

    YVMVQDMLSP SPMERPEAIN IIENAVFEDL DFPGKTVLRQ RSRSLSSSGT

    KHSRQSNNSH SPLPSN

  • UCSC uc002stc.5; human.;


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