Intended UseHuman E ELISA Kit allows for the in vitro quantitative determination of E , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntroduction: Estrogens are a group of steroid compounds, named for their importance in the estrous cycle, and functioning as the primary female sex hormone, their name comes from estrus/oistros (period of fertility for female mammals) + gen/gonos = to generate. Estrogens are used as part of some oral contraceptives, in estrogen replacement therapy for postmenopausal women, and in hormone replacement therapy for trans women. Like all steroid hormones, estrogens readily diffuse across the cell membrane. Once inside the cell, they bind to and activate estrogen receptors which in turn up-regulate the expression of many genes. Additionally, estrogens have been shown to activate a G protein-coupled receptor, GPR30.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Estrogen and Horseradish Peroxidase (HRP) conjugated Estrogen, then incubated. A competitive inhibition reaction is launched between Estrogen (Standards or samples) and Horseradish Peroxidase (HRP) conjugated Estrogen with the antibody specific for Estrogen. The more the amount of Estrogen in samples, the less antibody bound by Horseradish Peroxidase (HRP) conjugated Estrogen. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Estrogen in the sample. The color development is stopped and the intensity of the color is measured.