Human ERbeta ELISA Kit

Contact Us
  • Alternative name

    Estrogen receptor beta ELISA KIT; Nuclear receptor subfamily 3 group A member 2ESR2 ELISA KIT; NR3A2 ELISA KIT; ER-beta ELISA KIT

  • Catalog
    E013649
  • species
    Human
  • GeneERbeta
  • Standard CurveHuman ERbeta ELISA Kit
  • Other Species Mouse ERbeta ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ERbeta ELISA Kit allows for the in vitro quantitative determination of ERbeta , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: ESR2/ERb ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ESR2/ERb antibody and an ESR2/ERb-HRP conjugate. The assay sample and buffer are incubated together with ESR2/ERb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ESR2/ERb concentration since ESR2/ERb from samples and ESR2/ERb-HRP conjugate compete for the anti-ESR2/ERb antibody binding site. Since the number of sites is limited, as more sites are occupied by ESR2/ERb from the sample, fewer sites are left to bind ESR2/ERb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ESR2/ERb concentration in each sample is interpolated from this standard curve.



Inquiry About Human ERbeta ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!