Human ESR1 ELISA Kit

Alternative name
Human ER ELISA Kit;Human ER-alpha ELISA Kit;Human estradiol receptor ELISA Kit;Human nuclear receptor subfamily 3 group A member 1 ELISA Kit;Human ESR ELISA Kit;Human NR3A1 ELISA Kit;Human ESRA ELISA Kit;Human ESTRR ELISA Kit;Human Era ELISA Kit;Human estrogen receptor 1 ELISA Kit;Human estrogen receptor ELISA Kit;Human estrogen nuclear receptor alpha ELISA Kit;Human estrogen receptor alpha E1-E2-1-2 ELISA Kit;Human estrogen receptor alpha E1-N2-E2-1-2 ELISA Kit;
Catalog
E013639
species
Human
GeneESR1
Standard Curve
Other Species Mouse Esr1 ELISA Kit Bovine ESR1 ELISA Kit Chicken ESR1 ELISA Kit Horse ESR1 ELISA Kit Porcine ESR1 ELISA Kit Rat Esr1 ELISA Kit Sheep ESR1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intended Uses For detection of Estrogen receptor in human serum, plasma, urine, tissue samp
Intended UseHuman ESR1 ELISA Kit allows for the in vitro quantitative determination of ESR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEndocrinology ; Hormones; Oncology; Research Assays; Assay Categories
Product Description
specificalPrinciple of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-Estrogen receptor antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Estrogen receptor antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Estrogen receptor amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Estrogen receptor can be calculated. Background: The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen receptors are a group of proteins found inside cells. They are receptors that are activated by the hormone estrogen (17beta-estradiol). Metzger et al. (1988) demonstrated that the human estrogen receptor can be expressed in yeast. Once activated by estrogen, the estrogen receptor is able to bind to DNA and regulate the activity of many different genes (i.e. it is a DNA-binding transcription factor). However, it also has additional functions independent of DNA binding.
Human Estrogen receptor Protein information
Uniprot ID ESR1_HUMAN
Uniprot AC P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7;
UniGene Hs.208124; Hs.744830;
GeneID 2099
KEGG hsa:2099;
Human Estrogen receptor Protein SEQUENCE
SEQUENCE 595 AA; 66216 MW; 5455C57AB0CCCAA7 CRC64;
MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA
VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL
NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY
RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW
SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM
MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR
SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA
DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG
KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS
IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ
HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH
APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV
UCSC uc003qom.5; human. [P03372-1];

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