Human E3 ELISA Kit

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  • species
  • GeneE3
  • Standard CurveHuman E3 ELISA Kit
  • Other Species Human E3 ELISA KitHuman E3/UBPL ELISA KitMouse E3/UBPL ELISA KitMouse E3 ELISA KitBovine E3 ELISA KitBovine F-E3 ELISA KitCanine E3 ELISA KitCanine F-E3 ELISA KitChicken E3 ELISA KitChicken F-E3 ELISA KitHuman F-E3 ELISA KitGeneral E3 ELISA KitGeneral F-E3 ELISA KitMouse F-E3 ELISA KitPorcine E3 ELISA KitPorcine F-E3 ELISA KitRabbit E3 ELISA KitRabbit F-E3 ELISA KitRat E3 ELISA KitRat F-E3 ELISA KitCamel E3 ELISA KitCamel F-E3 ELISA KitGoat E3 ELISA KitGoat F-E3 ELISA KitGuinea Pig E3 ELISA KitGuinea Pig F-E3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman E3 ELISA Kit allows for the in vitro quantitative determination of E3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    Intended Uses: This E3 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofE3 in the sample, thisE3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusE3 concentration. The concentration ofE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This E3 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forE3. Standards or samples are then added to the microtiter plate wells andE3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofE3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forE3 are added to each well to "sandwich" theE3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containE3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

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