Human EPO ELISA Kit

Alternative name
Human EP ELISA Kit;Human MVCD2 ELISA Kit;Human erythropoietin ELISA Kit;Human epoetin ELISA Kit;
Catalog
E013577
species
Human
GeneEPO
Other Species Human EPO ab ELISA Kit Mouse EPO ab ELISA Kit Mouse EPO ELISA Kit Bovine EPO ELISA Kit Canine EPO ELISA Kit Horse EPO ELISA Kit Monkey EPO ELISA Kit Porcine EPO ELISA Kit Rabbit EPO ELISA Kit Sheep EPO ELISA Kit
SpecificityCross-reactivity in the EPO was studied by the addition of various substances to the Zero Calibrator (Calibrator A).
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe sensitivity, or minimum detection limit, of this assay is defined as the smallest single value, which can be distinguished from zero at the 95% confidence limit. The EPO ELISA has a calculated sensitivity of 2.0 mU/mL. Hence, patient sample results be
Intended UseHuman EPO ELISA Kit allows for the in vitro quantitative determination of EPO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCytokines & Cell Signalling; Endocrinology ; Hormones; Diagnostic Assays; Assay Categories
Product Description
specificalPrinciple of the assay: The EPO Immunoassay is a two-site ELISA [Enzyme-Linked ImmunoSorbent Assay] for the measurement of the biologically active 165 amino acid chain of EPO. A sheep polyclonal antibody to human EPO, purified by affinity chromatography, is biotinylated. A mouse monoclonal antibody to human EPO is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of EPO in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of EPO present in the controls and patient samples are determined directly from this curve. The standards have been calibrated against the World Health Organization (WHO) erythropoietin international standard which consists of recombinant DNA derived EPO. The WHO reference standard used was erythropoietin 1st international standard (87/684).
Human Erythropoietin Protein information
Uniprot ID EPO_HUMAN
Uniprot AC P01588; Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0;
UniGene Hs.2303;
GeneID 2056
KEGG hsa:2056;
Human Erythropoietin Protein SEQUENCE
SEQUENCE 193 AA; 21307 MW; C91F0E4C26A52033 CRC64;
MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE
NITTGCAEHC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA
VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD
AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR
UCSC uc003uwi.5; human.;

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Category

Human EPO ELISA Kit

Human EP ELISA Kit;Human MVCD2 ELISA Kit;Human erythropoietin ELISA Kit;Human epoetin ELISA Kit;