Human EPB42 ELISA Kit

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  • Alternative name

    Human erythrocyte protein 4.2 ELISA Kit;Human P4.2 ELISA Kit;Human E42P ELISA Kit;Human PA ELISA Kit;Human SPH5 ELISA Kit;Human erythrocyte membrane protein band 4.2 ELISA Kit;Human erythrocyte surface protein band 4.2 ELISA Kit;

  • Catalog
    E013568
  • species
    Human
  • GeneEPB42
  • Standard CurveHuman EPB42 ELISA Kit
  • Other Species Mouse Epb42 ELISA KitBovine EPB42 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Erythrocyte Membrane Protein Band 4.2 (EPB42). No significant cross-reactivity or interference between Erythrocyte Membrane Protein Band 4.2 (EPB42) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman EPB42 ELISA Kit allows for the in vitro quantitative determination of EPB42 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythrocyte Membrane Protein Band 4.2 (EPB42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythrocyte Membrane Protein Band 4.2 (EPB42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Erythrocyte membrane protein band 4.2 Protein information
  • Uniprot ID EPB42_HUMAN
  • Uniprot AC P16452; Q4KKX0; Q4VB97;
  • UniGene Hs.368642;
  • GeneID 2038
  • KEGG hsa:2038;
  • Human Erythrocyte membrane protein band 4.2 Protein SEQUENCE
  • SEQUENCE 691 AA; 77009 MW; 38225C311E478580 CRC64;

    MGQALGIKSC DFQAARNNEE HHTKALSSRR LFVRRGQPFT IILYFRAPVR

    AFLPALKKVA LTAQTGEQPS KINRTQATFP ISSLGDRKWW SAVVEERDAQ

    SWTISVTTPA DAVIGHYSLL LQVSGRKQLL LGQFTLLFNP WNREDAVFLK

    NEAQRMEYLL NQNGLIYLGT ADCIQAESWD FGQFEGDVID LSLRLLSKDK

    QVEKWSQPVH VARVLGALLH FLKEQRVLPT PQTQATQEGA LLNKRRGSVP

    ILRQWLTGRG RPVYDGQAWV LAAVACTVLR CLGIPARVVT TFASAQGTGG

    RLLIDEYYNE EGLQNGEGQR GRIWIFQTST ECWMTRPALP QGYDGWQILH

    PSAPNGGGVL GSCDLVPVRA VKEGTLGLTP AVSDLFAAIN ASCVVWKCCE

    DGTLELTDSN TKYVGNNIST KGVGSDRCED ITQNYKYPEG SLQEKEVLER

    VEKEKMEREK DNGIRPPSLE TASPLYLLLK APSSLPLRGD AQISVTLVNH

    SEQEKAVQLA IGVQAVHYNG VLAAKLWRKK LHLTLSANLE KIITIGLFFS

    NFERNPPENT FLRLTAMATH SESNLSCFAQ EDIAICRPHL AIKMPEKAEQ

    YQPLTASVSL QNSLDAPMED CVISILGRGL IHRERSYRFR SVWPENTMCA

    KFQFTPTHVG LQRLTVEVDC NMFQNLTNYK SVTVVAPELS A

  • UCSC uc001zra.5; human. [P16452-1];




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