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Human ERBB3 ELISA Kit

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  • Alternative name

    Human proto-oncogene-like protein c-ErbB-3 ELISA Kit;Human tyrosine kinase-type cell surface receptor HER3 ELISA Kit;Human HER3 ELISA Kit;Human ErbB-3 ELISA Kit;Human LCCS2 ELISA Kit;Human MDA-BF-1 ELISA Kit;Human c-erbB-3 ELISA Kit;Human c-erbB3 ELISA Kit;Human erbB3-S ELISA Kit;Human p180-ErbB3 ELISA Kit;Human p45-sErbB3 ELISA Kit;Human p85-sErbB3 ELISA Kit;Human erb-b2 receptor tyrosine kinase 3 ELISA Kit;Human receptor tyrosine-protein kinase erbB-3 ELISA Kit;Human human epidermal growth factor receptor 3 ELISA Kit;Human v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 ELISA Kit;
  • Catalog
    E013538
  • species
    Human
  • GeneERBB3
  • Standard CurveHuman ERBB3 ELISA Kit
  • Other Species Mouse Erbb3 ELISA KitRat Erbb3 ELISA Kit
  • SpecificityNatural and recombinant human ERBB3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human ERBB3 in cell culture supernates, serum and plas
  • Intended UseHuman ERBB3 ELISA Kit allows for the in vitro quantitative determination of ERBB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: Human ERBB3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ERBB3 has been precoated onto 96-well plates. Standards (NSO, S20-T643) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ERBB3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ERBB3 amount of sample captured in plate. Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.
  • Human Receptor tyrosine-protein kinase erbB-3 Protein information
  • Uniprot ID ERBB3_HUMAN
  • Uniprot AC P21860; A8K6L6; B4DIK7; B4DV32; E9PDT8; Q9BUD7;
  • UniGene Hs.118681; Hs.622058;
  • GeneID 2065
  • KEGG hsa:2065;
  • Human Receptor tyrosine-protein kinase erbB-3 Protein SEQUENCE

  • SEQUENCE 1342 AA; 148098 MW; 7201E7F66CA374BD CRC64;

    MRANDALQVL GLLFSLARGS EVGNSQAVCP GTLNGLSVTG DAENQYQTLY

    KLYERCEVVM GNLEIVLTGH NADLSFLQWI REVTGYVLVA MNEFSTLPLP

    NLRVVRGTQV YDGKFAIFVM LNYNTNSSHA LRQLRLTQLT EILSGGVYIE

    KNDKLCHMDT IDWRDIVRDR DAEIVVKDNG RSCPPCHEVC KGRCWGPGSE

    DCQTLTKTIC APQCNGHCFG PNPNQCCHDE CAGGCSGPQD TDCFACRHFN

    DSGACVPRCP QPLVYNKLTF QLEPNPHTKY QYGGVCVASC PHNFVVDQTS

    CVRACPPDKM EVDKNGLKMC EPCGGLCPKA CEGTGSGSRF QTVDSSNIDG

    FVNCTKILGN LDFLITGLNG DPWHKIPALD PEKLNVFRTV REITGYLNIQ

    SWPPHMHNFS VFSNLTTIGG RSLYNRGFSL LIMKNLNVTS LGFRSLKEIS

    AGRIYISANR QLCYHHSLNW TKVLRGPTEE RLDIKHNRPR RDCVAEGKVC

    DPLCSSGGCW GPGPGQCLSC RNYSRGGVCV THCNFLNGEP REFAHEAECF

    SCHPECQPME GTATCNGSGS DTCAQCAHFR DGPHCVSSCP HGVLGAKGPI

    YKYPDVQNEC RPCHENCTQG CKGPELQDCL GQTLVLIGKT HLTMALTVIA

    GLVVIFMMLG GTFLYWRGRR IQNKRAMRRY LERGESIEPL DPSEKANKVL

    ARIFKETELR KLKVLGSGVF GTVHKGVWIP EGESIKIPVC IKVIEDKSGR

    QSFQAVTDHM LAIGSLDHAH IVRLLGLCPG SSLQLVTQYL PLGSLLDHVR

    QHRGALGPQL LLNWGVQIAK GMYYLEEHGM VHRNLAARNV LLKSPSQVQV

    ADFGVADLLP PDDKQLLYSE AKTPIKWMAL ESIHFGKYTH QSDVWSYGVT

    VWELMTFGAE PYAGLRLAEV PDLLEKGERL AQPQICTIDV YMVMVKCWMI

    DENIRPTFKE LANEFTRMAR DPPRYLVIKR ESGPGIAPGP EPHGLTNKKL

    EEVELEPELD LDLDLEAEED NLATTTLGSA LSLPVGTLNR PRGSQSLLSP

    SSGYMPMNQG NLGESCQESA VSGSSERCPR PVSLHPMPRG CLASESSEGH

    VTGSEAELQE KVSMCRSRSR SRSPRPRGDS AYHSQRHSLL TPVTPLSPPG

    LEEEDVNGYV MPDTHLKGTP SSREGTLSSV GLSSVLGTEE EDEDEEYEYM

    NRRRRHSPPH PPRPSSLEEL GYEYMDVGSD LSASLGSTQS CPLHPVPIMP

    TAGTTPDEDY EYMNRQRDGG GPGGDYAAMG ACPASEQGYE EMRAFQGPGH

    QAPHVHYARL KTLRSLEATD SAFDNPDYWH SRLFPKANAQ RT

  • UCSC uc001sjg.4; human. [P21860-1];


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