Human EPR ELISA Kit;Human ER ELISA Kit;Human Ep ELISA Kit;Human epiregulin ELISA Kit;Human proepiregulin ELISA Kit;
Catalog
E013437
species
Human
GeneEREG
SpecificityThis assay has high sensitivity and excellent specificity for detection of Epiregulin (EREG).
No significant cross-reactivity or interference between Epiregulin (EREG) and analogues was observed.
Intended UseHuman EREG ELISA Kit allows for the in vitro quantitative determination of EREG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epiregulin (EREG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epiregulin (EREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epiregulin (EREG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epiregulin (EREG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.