Human LCN12 ELISA Kit

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  • Alternative name

    Human lipocalin 12 ELISA Kit;Human epididymal-specific lipocalin-12 ELISA Kit;Human lipocalcin 12 ELISA Kit;

  • Catalog
    E013413
  • species
    Human
  • GeneLCN12
  • Other Species Mouse Lcn12 ELISA KitRat Lcn12 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 37.5pg/ml
  • Intended UseHuman LCN12 ELISA Kit allows for the in vitro quantitative determination of LCN12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LCN12 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LCN12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LCN12 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LCN12can be calculated.
  • Human Epididymal-specific lipocalin-12 Protein information
  • Uniprot ID LCN12_HUMAN
  • Uniprot AC Q6JVE5; A2AMJ7;
  • UniGene Hs.440519;
  • GeneID 286256
  • KEGG hsa:286256;
  • Human Epididymal-specific lipocalin-12 Protein SEQUENCE
  • SEQUENCE 192 AA; 21500 MW; F4681F87CCF7A230 CRC64;

    MRLLCGLWLW LSLLKVLQAQ TPTPLPLPPP MQSFQGNQFQ GEWFVLGLAG

    NSFRPEHRAL LNAFTATFEL SDDGRFEVWN AMTRGQHCDT WSYVLIPAAQ

    PGQFTVDHGV EPGADREETR VVDSDYTQFA LMLSRRHTSR LAVLRISLLG

    RSWLLPPGTL DQFICLGRAQ GLSDDNIVFP DVTGWSPQAS VC

  • UCSC uc004ckb.4; human.;



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