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  • Alternative name

    Receptor tyrosine-protein kinase erbB-2 ELISA KIT; Metastatic lymph node gene 19 protein ELISA KIT; MLN 19 ELISA KIT; Proto-oncogene Neu ELISA KIT; Proto-oncogene c-ErbB-2 ELISA KIT; Tyrosine kinase-type cell surface receptor HER2 ELISA KIT; p185erbB2 ELISA KIT; CD_antigen: CD340ERBB2 ELISA KIT; HER2 ELISA KIT; MLN19 ELISA KIT; NEU ELISA KIT; NGL ELISA KIT; MLN 19 ELISA KIT

  • Catalog
  • species
  • GeneHER 2 ECD
  • Other Species Human HER-2 ECD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EGFR2ED. No significant cross-reactivity or interference between EGFR2ED and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EGFR2ED and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HER 2 ECD ELISA Kit allows for the in vitro quantitative determination of HER 2 ECD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This EGFR2ED ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EGFR2ED. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||EGFR2ED ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EGFR2ED. Standards or samples are then added to the microtiter plate wells and EGFR2ED if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EGFR2ED present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EGFR2ED are added to each well to "sandwich" the EGFR2ED immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGFR2ED and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFR2ED concentration in each sample is interpolated from this standard curve.

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