Human EPCAM ELISA Kit

Alternative name
Human Ep-CAM ELISA Kit;Human adenocarcinoma-associated antigen ELISA Kit;Human cell surface glycoprotein Trop-1 ELISA Kit;Human Epithelial cell surface antigen ELISA Kit;Human Epithelial glycoprotein ELISA Kit;Human EGP ELISA Kit;Human epithelial glycoprotein 314 ELISA Kit;Human EGP314 ELISA Kit;Human hEGP314 ELISA Kit;Human KS 1/4 antigen ELISA Kit;Human KSA ELISA Kit;Human major gastrointestinal tumor-associated protein GA733-2 ELISA Kit;Human tumor-associated calcium signal transducer 1 ELISA Kit;Human GA733-2 ELISA Kit;Human M1S2 ELISA Kit;Human M4S1 ELISA Kit;Human MIC18 ELISA Kit;Human TACSTD1 ELISA Kit;Human TROP1 ELISA Kit;Human DIAR5 ELISA Kit;Human EGP-2 ELISA Kit;Human EGP40 ELISA Kit;Human ESA ELISA Kit;Human HNPCC8 ELISA Kit;Human KS1/4 ELISA Kit;Human MK-1 ELISA Kit;Human epithelial cell adhesion molecule ELISA Kit;Human human epithelial glycoprotein-2 ELISA Kit;Human membrane component, chromosome 4, surface marker (35kD glycoprotein) ELISA Kit;
Catalog
E013287
species
Human
GeneEPCAM
Standard Curve
Other Species Mouse Epcam ELISA Kit Bovine EPCAM ELISA Kit Chicken EPCAM ELISA Kit Rat Epcam ELISA Kit
SpecificityNatural and recombinant human EPCAM
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human EPCAM in cell culture supernates, serum and plas
Intended UseHuman EPCAM ELISA Kit allows for the in vitro quantitative determination of EPCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human EPCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCAM has been precoated onto 96-well plates. Standards(NSO, Q24 - K265) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCAM amount of sample captured in plate. Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
Human Epithelial cell adhesion molecule Protein information
Uniprot ID EPCAM_HUMAN
Uniprot AC P16422; P18180; Q6FG26; Q6FG49; Q96C47; Q9UCD0;
UniGene Hs.542050;
GeneID 4072
KEGG hsa:4072;
Human Epithelial cell adhesion molecule Protein SEQUENCE
SEQUENCE 314 AA; 34932 MW; 023FCE418B2F1079 CRC64;
MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS
VGAQNTVICS KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD
ESGLFKAKQC NGTSMCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH
KAREKPYDSK SLRTALQKEI TTRYQLDPKF ITSILYENNV ITIDLVQNSS
QKTQNDVDIA DVAYYFEKDV KGESLFHSKK MDLTVNGEQL DLDPGQTLIY
YVDEKAPEFS MQGLKAGVIA VIVVVVIAVV AGIVVLVISR KKRMAKYEKA
EIKEMGEMHR ELNA
UCSC uc002rvx.4; human.;

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Human EPCAM ELISA Kit