Human AKR1C4 ELISA Kit

Alternative name
Human 3-alpha-HSD1 ELISA Kit;Human 3-alpha-hydroxysteroid dehydrogenase type I ELISA Kit;Human Chlordecone reductase ELISA Kit;Human CDR ELISA Kit;Human Dihydrodiol dehydrogenase 4 ELISA Kit;Human DD-4 ELISA Kit;Human DD4 ELISA Kit;Human HAKRA ELISA Kit;Human CHDR ELISA Kit;Human 3-alpha-HSD ELISA Kit;Human C11 ELISA Kit;Human aldo-keto reductase family 1 member C4 ELISA Kit;Human chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4 ELISA Kit;Human dihydrodiol dehydrogenase isozyme DD4 ELISA Kit;Human type I 3-alpha-hydroxysteroid dehydrogenase ELISA Kit;
Catalog
E001323
species
Human
GeneAKR1C4
Standard Curve
Other Species Monkey AKR1C4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKR1C4. No significant cross-reactivity or interference between AKR1C4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR1C4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AKR1C4 ELISA Kit allows for the in vitro quantitative determination of AKR1C4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: AKR1C4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR1C4 antibody and an AKR1C4-HRP conjugate. The assay sample and buffer are incubated together with AKR1C4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1C4 concentration since AKR1C4 from samples and AKR1C4-HRP conjugate compete for the anti-AKR1C4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1C4 from the sample, fewer sites are left to bind AKR1C4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1C4 concentration in each sample is interpolated from this standard curve.
Human Aldo-keto reductase family 1 member C4 Protein information
Uniprot ID AK1C4_HUMAN
Uniprot AC P17516; Q5T6A3; Q8WW84; Q9NS54;
UniGene Hs.567245;
GeneID 1109
KEGG hsa:1109;
Human Aldo-keto reductase family 1 member C4 Protein SEQUENCE
SEQUENCE 323 AA; 37067 MW; E728CE4B420E8C58 CRC64;
MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID
SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE
SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV
MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS
KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKQ
TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN
RNYRYVVMDF LMDHPDYPFS DEY
UCSC uc001ihw.2; human.;

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