Human EVPL ELISA Kit

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  • Alternative name

    Human 210 kDa cornified envelope precursor protein ELISA Kit;Human 210 kDa paraneoplastic pemphigus antigen ELISA Kit;Human p210 ELISA Kit;Human EVPK ELISA Kit;Human envoplakin ELISA Kit;

  • Catalog
    E013227
  • species
    Human
  • GeneEVPL
  • Standard CurveHuman EVPL ELISA Kit
  • Other Species Mouse Evpl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Envoplakin (EVPL). No significant cross-reactivity or interference between Envoplakin (EVPL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman EVPL ELISA Kit allows for the in vitro quantitative determination of EVPL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Envoplakin (EVPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Envoplakin (EVPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Envoplakin (EVPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Envoplakin (EVPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Envoplakin Protein information
  • Uniprot ID EVPL_HUMAN
  • Uniprot AC Q92817; A0AUV5;
  • UniGene Hs.500635;
  • GeneID 2125
  • KEGG hsa:2125;
  • Human Envoplakin Protein SEQUENCE
  • SEQUENCE 2033 AA; 231604 MW; 0A994627703393E0 CRC64;

    MFKGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA

    DQVERDILET QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD

    VDKARRLKHP QAEEIEKDIK QLHERVTQEC AEYRALYEKM VLPPDVGPRV

    DWARVLEQKQ KQVCAGQYGP GMAELEQQIA EHNILQKEID AYGQQLRSLV

    GPDAATIRSQ YRDLLKAASW RGQSLGSLYT HLQGCTRQLS ALAEQQRRIL

    QQDWSDLMAD PAGVRREYEH FKQHELLSQE QSVNQLEDDG ERMVELRHPA

    VGPIQAHQEA LKMEWQNFLN LCICQETQLQ HVEDYRRFQE EADSVSQTLA

    KLNSNLDAKY SPAPGGPPGA PTELLQQLEA EEKRLAVTER ATGDLQRRSR

    DVAPLPQRRN PPQQPLHVDS ICDWDSGEVQ LLQGERYKLV DNTDPHAWVV

    QGPGGETKRA PAACFCIPAP DPDAVARASR LASELQALKQ KLATVQSRLK

    ASAVESLRPS QQAPSGSDLA NPQAQKLLTQ MTRLDGDLGQ IERQVLAWAR

    APLSRPTPLE DLEGRIHSHE GTAQRLQSLG TEKETAQKEC EAFLSTRPVG

    PAALQLPVAL NSVKNKFSDV QVLCSLYGEK AKAALDLERQ IQDADRVIRG

    FEATLVQEAP IPAEPGALQE RVSELQRQRR ELLEQQTCVL RLHRALKASE

    HACAALQNNF QEFCQDLPRQ QRQVRALTDR YHAVGDQLDL REKVVQDAAL

    TYQQFKNCKD NLSSWLEHLP RSQVRPSDGP SQIAYKLQAQ KRLTQEIQSR

    ERDRATASHL SQALQAALQD YELQADTYRC SLEPTLAVSA PKRPRVAPLQ

    ESIQAQEKNL AKAYTEVAAA QQQLLQQLEF ARKMLEKKEL SEDIRRTHDA

    KQGSESPAQA GRESEALKAQ LEEERKRVAR VQHELEAQRS QLLQLRTQRP

    LERLEEKEVV EFYRDPQLEG SLSRVKAQVE EEGKRRAGLQ ADLEVAAQKV

    VQLESKRKTM QPHLLTKEVT QVERDPGLDS QAAQLRIQIQ QLRGEDAVIS

    ARLEGLKKEL LALEKREVDV KEKVVVKEVV KVEKNLEMVK AAQALRLQME

    EDAARRKQAE EAVAKLQARI EDLERAISSV EPKVIVKEVK KVEQDPGLLQ

    ESSRLRSLLE EERTKNATLA RELSDLHSKY SVVEKQRPKV QLQERVHEIF

    QVDPETEQEI TRLKAKLQEM AGKRSGVEKE VEKLLPDLEV LRAQKPTVEY

    KEVTQEVVRH ERSPEVLREI DRLKAQLNEL VNSHGRSQEQ LIRLQGERDE

    WRRERAKVET KTVSKEVVRH EKDPVLEKEA ERLRQEVREA AQKRRAAEDA

    VYELQSKRLL LERRKPEEKV VVQEVVVTQK DPKLREEHSR LSGSLDEEVG

    RRRQLELEVQ QLRAGVEEQE GLLSFQEDRS KKLAVERELR QLTLRIQELE

    KRPPTVQEKI IMEEVVKLEK DPDLEKSTEA LRWDLDQEKT QVTELNRECK

    NLQVQIDVLQ KAKSQEKTIY KEVIRVQKDR VLEDERARVW EMLNRERTAR

    QAREEEARRL RERIDRAETL GRTWSREESE LQRARDQADQ ECGRLQQELR

    ALERQKQQQT LQLQEESKLL SQKTESERQK AAQRGQELSR LEAAILREKD

    QIYEKERTLR DLHAKVSREE LSQETQTRET NLSTKISILE PETGKDMSPY

    EAYKRGIIDR GQYLQLQELE CDWEEVTTSG PCGEESVLLD RKSGKQYSIE

    AALRCRRISK EEYHLYKDGH LPISEFALLV AGETKPSSSL SIGSIISKSP

    LASPAPQSTS FFSPSFSLGL GDDSFPIAGI YDTTTDNKCS IKTAVAKNML

    DPITGQKLLE AQAATGGIVD LLSRERYSVH KAMERGLIEN TSTQRLLNAQ

    KAFTGIEDPV TKKRLSVGEA VQKGWMPRES VLPHLQVQHL TGGLIDPKRT

    GRIPIQQALL SGMISEELAQ LLQDESSYEK DLTDPISKER LSYKEAMGRC

    RKDPLSGLLL LPAALEGYRC YRSASPTVPR SLR

  • UCSC uc002jqi.3; human.;


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