Human AKR1C3 ELISA Kit

Alternative name
Human 17-beta-hydroxysteroid dehydrogenase type 5 ELISA Kit;Human 17-beta-HSD 5 ELISA Kit;Human 3-alpha-HSD type II, brain ELISA Kit;Human 3-alpha-hydroxysteroid dehydrogenase type 2 ELISA Kit;Human 3-alpha-HSD type 2 ELISA Kit;Human chlordecone reductase homolog HAKRb ELISA Kit;Human dihydrodiol dehydrogenase 3 ELISA Kit;Human DD-3 ELISA Kit;Human DD3 ELISA Kit;Human Dihydrodiol dehydrogenase type I ELISA Kit;Human HA1753 ELISA Kit;Human indanol dehydrogenase ELISA Kit;Human prostaglandin F synthase ELISA Kit;Human PGFS ELISA Kit;Human testosterone 17-beta-dehydrogenase 5 ELISA Kit;Human trans-1,2-dihydrobenzene-1,2-diol dehydrogenase ELISA Kit;Human DDH1 ELISA Kit;Human HSD17B5 ELISA Kit;Human KIAA0119 ELISA Kit;Human DDX ELISA Kit;Human HAKRB ELISA Kit;Human HAKRe ELISA Kit;Human hluPGFS ELISA Kit;Human aldo-keto reductase family 1 member C3 ELISA Kit;Human 3-alpha hydroxysteroid dehydrogenase, type II ELISA Kit;Human dihydrodiol dehydrogenase X ELISA Kit;Human type IIb 3-alpha hydroxysteroid dehydrogenase ELISA Kit;
Catalog
E001322
species
Human
GeneAKR1C3
Standard Curve
Other Species Mouse AKR1C3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKR1C3. No significant cross-reactivity or interference between AKR1C3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR1C3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman AKR1C3 ELISA Kit allows for the in vitro quantitative determination of AKR1C3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: AKR1C3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR1C3 antibody and an AKR1C3-HRP conjugate. The assay sample and buffer are incubated together with AKR1C3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1C3 concentration since AKR1C3 from samples and AKR1C3-HRP conjugate compete for the anti-AKR1C3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1C3 from the sample, fewer sites are left to bind AKR1C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1C3 concentration in each sample is interpolated from this standard curve.
Human Aldo-keto reductase family 1 member C3 Protein information
Uniprot ID AK1C3_HUMAN
Uniprot AC Q9UII3; Q9UKL9;
UniGene Hs.78183;
GeneID 8644
KEGG hsa:8644;
Human Aldo-keto reductase family 1 member C3 Protein SEQUENCE
SEQUENCE 323 AA; 36853 MW; 86A7690D9498C6FD CRC64;
MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID
SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE
NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA
MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS
KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR
TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD
RNLHYFNSDS FASHPNYPYS DEY
UCSC uc001ihu.4; human. [P42330-1];

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