Human ENO2 ELISA Kit

Alternative name
Human 2-phospho-D-glycerate hydro-lyase ELISA Kit;Human enolase 2 ELISA Kit;Human neural enolase ELISA Kit;Human neuron-specific enolase ELISA Kit;Human NSE ELISA Kit;Human HEL-S-279 ELISA Kit;Human gamma-enolase ELISA Kit;Human 2-phospho-D-glycerate hydrolyase ELISA Kit;Human enolase 2 (gamma, neuronal) ELISA Kit;Human epididymis secretory protein Li 279 ELISA Kit;Human neuron specific gamma enolase ELISA Kit;Human neuronal enriched enolase ELISA Kit;Human neurone-specific enolase ELISA Kit;
Catalog
E013196
species
Human
GeneENO2
Other Species Mouse Eno2 ELISA Kit Chicken ENO2 ELISA Kit Rat Eno2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ENO2. No significant cross-reactivity or interference between Human ENO2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.2 ng/ml
Intended UseHuman ENO2 ELISA Kit allows for the in vitro quantitative determination of ENO2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Neuron Specific Enolase (NSE) is the most abundant form of the glycolytic enolase found in adult neurons and is thought to serve as a growth factor in neurons. Of the three enolase subunits (alpha, beta, and gamma), NSE is a dimer composed of two gamma subunits. NSE is useful in studying neuronal differentiation and is, therefore, a valuable tool for visualizing the entire neuron and endocrine systems. Serum levels of NSE have been associated with such disease states as Alzheimer's, Huntington's Chorea, neuroblastoma, head trauma, neuroendocrine malignancies, and small cell carcinomas of the lung. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for NSE to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NSE, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Gamma-enolase Protein information
Uniprot ID ENOG_HUMAN
Uniprot AC P09104; B7Z2X9; Q96J33;
UniGene Hs.511915;
GeneID 2026
KEGG hsa:2026;
Human Gamma-enolase Protein SEQUENCE
SEQUENCE 434 AA; 47269 MW; 6163DE81F5C67744 CRC64;
MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR
DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT
ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF
NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY
GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE
FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD
WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV
TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR
SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
UCSC uc058knm.1; human. [P09104-1];

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Human ENO2 ELISA Kit