Human EDN3 ELISA Kit

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  • Alternative name

    Human ET-3 ELISA Kit;Human preproendothelin-3 ELISA Kit;Human PPET3 ELISA Kit;Human ET3 ELISA Kit;Human HSCR4 ELISA Kit;Human WS4B ELISA Kit;Human endothelin 3 ELISA Kit;Human endothelin-3 ELISA Kit;

  • Catalog
    E013113
  • species
    Human
  • GeneEDN3
  • Other Species Mouse Edn3 ELISA KitCanine EDN3 ELISA KitPorcine EDN3 ELISA KitRabbit EDN3 ELISA KitRat Edn3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human EDN3. No significant cross-reactivity or interference between human EDN3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human EDN3 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman EDN3 ELISA Kit allows for the in vitro quantitative determination of EDN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDN3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Endothelin-3 Protein information
  • Uniprot ID EDN3_HUMAN
  • Uniprot AC P14138; E1P5I5; Q03229; Q7Z6D2; Q9UGT7;
  • UniGene Hs.1408;
  • GeneID 1908
  • KEGG hsa:1908;
  • Human Endothelin-3 Protein SEQUENCE
  • SEQUENCE 238 AA; 25454 MW; 7D7E90BC7DE37273 CRC64;

    MEPGLWLLFG LTVTSAAGFV PCSQSGDAGR RGVSQAPTAA RSEGDCEETV

    AGPGEETVAG PGEGTVAPTA LQGPSPGSPG QEQAAEGAPE HHRSRRCTCF

    TYKDKECVYY CHLDIIWINT PEQTVPYGLS NYRGSFRGKR SAGPLPGNLQ

    LSHRPHLRCA CVGRYDKACL HFCTQTLDVS SNSRTAEKTD KEEEGKVEVK

    DQQSKQALDL HHPKLMPGSG LALAPSTCPR CLFQEGAP

  • UCSC uc002yap.4; human. [P14138-1];



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