Human EDN1 ELISA Kit

Alternative name
Human preproendothelin-1 ELISA Kit;Human PPET1 ELISA Kit;Human ET-1 ELISA Kit;Human ARCND3 ELISA Kit;Human ET1 ELISA Kit;Human HDLCQ7 ELISA Kit;Human QME ELISA Kit;Human endothelin 1 ELISA Kit;Human endothelin-1 ELISA Kit;
Catalog
E013102
species
Human
GeneEDN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ET-1. No significant cross-reactivity or interference between ET-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ET-1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman EDN1 ELISA Kit allows for the in vitro quantitative determination of EDN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: ET-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ET-1. Standards or samples are then added to the microtiter plate wells and ET-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ET-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ET-1 are added to each well to "sandwich" the ET-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ET-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET-1 concentration in each sample is interpolated from this standard curve.
Human Big endothelin-1 Protein information
Uniprot ID EDN1_HUMAN
Uniprot AC P05305; Q96DA1;
UniGene Hs.511899; Hs.713645;
GeneID 1906
KEGG hsa:1906;
Human Big endothelin-1 Protein SEQUENCE
SEQUENCE 212 AA; 24425 MW; E2DA0EB86755BC3D CRC64;
MDYLLMIFSL LFVACQGAPE TAVLGAELSA VGENGGEKPT PSPPWRLRRS
KRCSCSSLMD KECVYFCHLD IIWVNTPEHV VPYGLGSPRS KRALENLLPT
KATDRENRCQ CASQKDKKCW NFCQAGKELR AEDIMEKDWN NHKKGKDCSK
LGKKCIYQQL VRGRKIRRSS EEHLRQTRSE TMRNSVKSSF HDPKLKGKPS
RERYVTHNRA HW

Category

Human EDN1 ELISA Kit

Human preproendothelin-1 ELISA Kit;Human PPET1 ELISA Kit;Human ET-1 ELISA Kit;Human ARCND3 ELISA Kit;Human ET1 ELISA Kit;Human HDLCQ7 ELISA Kit;Human QME ELISA Kit;Human endothelin 1 ELISA Kit;Human endothelin-1 ELISA Kit;