Human COL18A1 ELISA Kit

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  • Alternative name

    Human KNO ELISA Kit;Human KNO1 ELISA Kit;Human KS ELISA Kit;Human collagen type XVIII alpha 1 chain ELISA Kit;Human collagen alpha-1(XVIII) chain ELISA Kit;Human antiangiogenic agent ELISA Kit;Human collagen alpha-1(XVIII) chain isoform 1 preproprotein ELISA Kit;Human collagen, type XVIII, alpha 1 ELISA Kit;Human endostatin ELISA Kit;Human multi-functional protein MFP ELISA Kit;

  • Catalog
    E013065
  • species
    Human
  • GeneCOL18A1
  • Standard CurveHuman COL18A1 ELISA Kit
  • Other Species Mouse Col18a1 ELISA KitMouse COL18A1/ES ELISA KitMouse Col17a1 ELISA Kit
  • SpecificityNatural and recombinant human Endostatin
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman COL18A1 ELISA Kit allows for the in vitro quantitative determination of COL18A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from human specific for Endostatin has been precoated onto 96-well plates. Standards(E.coli, H1154-S1335) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Endostatin amount of sample captured in plate. Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar toangiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
  • Human Endostatin Protein information
  • Uniprot ID COIA1_HUMAN
  • Uniprot AC Q96T70; Q9UK38; Q9Y6Q7; Q9Y6Q8;
  • UniGene Hs.517356;
  • GeneID 80781
  • KEGG hsa:80781;
  • Human Endostatin Protein SEQUENCE
  • SEQUENCE 1754 AA; 178188 MW; 23A327DCBD3B328D CRC64;

    MAPYPCGCHI LLLLFCCLAA ARANLLNLNW LWFNNEDTSH AATTIPEPQG

    PLPVQPTADT TTHVTPRNGS TEPATAPGSP EPPSELLEDG QDTPTSAESP

    DAPEENIAGV GAEILNVAKG IRSFVQLWND TVPTESLARA ETLVLETPVG

    PLALAGPSST PQENGTTLWP SRGIPSSPGA HTTEAGTLPA PTPSPPSLGR

    PWAPLTGPSV PPPSSGRASL SSLLGGAPPW GSLQDPDSQG LSPAAAAPSQ

    QLQRPDVRLR TPLLHPLVMG SLGKHAAPSA FSSGLPGALS QVAVTTLTRD

    SGAWVSHVAN SVGPGLANNS ALLGADPEAP AGRCLPLPPS LPVCGHLGIS

    RFWLPNHLHH ESGEQVRAGA RAWGGLLQTH CHPFLAWFFC LLLVPPCGSV

    PPPAPPPCCQ FCEALQDACW SRLGGGRLPV ACASLPTQED GYCVLIGPAA

    ERISEEVGLL QLLGDPPPQQ VTQTDDPDVG LAYVFGPDAN SGQVARYHFP

    SLFFRDFSLL FHIRPATEGP GVLFAITDSA QAMVLLGVKL SGVQDGHQDI

    SLLYTEPGAG QTHTAASFRL PAFVGQWTHL ALSVAGGFVA LYVDCEEFQR

    MPLARSSRGL ELEPGAGLFV AQAGGADPDK FQGVIAELKV RRDPQVSPMH

    CLDEEGDDSD GASGDSGSGL GDARELLREE TGAALKPRLP APPPVTTPPL

    AGGSSTEDSR SEEVEEQTTV ASLGAQTLPG SDSVSTWDGS VRTPGGRVKE

    GGLKGQKGEP GVPGPPGRAG PPGSPCLPGP PGLPCPVSPL GPAGPALQTV

    PGPQGPPGPP GRDGTPGRDG EPGDPGEDGK PGDTGPQGFP GTPGDVGPKG

    DKGDPGVGER GPPGPQGPPG PPGPSFRHDK LTFIDMEGSG FGGDLEALRG

    PRGFPGPPGP PGVPGLPGEP GRFGVNSSDV PGPAGLPGVP GREGPPGFPG

    LPGPPGPPGR EGPPGRTGQK GSLGEAGAPG HKGSKGAPGP AGARGESGLA

    GAPGPAGPPG PPGPPGPPGP GLPAGFDDME GSGGPFWSTA RSADGPQGPP

    GLPGLKGDPG VPGLPGAKGE VGADGVPGFP GLPGREGIAG PQGPKGDRGS

    RGEKGDPGKD GVGQPGLPGP PGPPGPVVYV SEQDGSVLSV PGPEGRPGFA

    GFPGPAGPKG NLGSKGERGS PGPKGEKGEP GSIFSPDGGA LGPAQKGAKG

    EPGFRGPPGP YGRPGYKGEI GFPGRPGRPG MNGLKGEKGE PGDASLGFGM

    RGMPGPPGPP GPPGPPGTPV YDSNVFAESS RPGPPGLPGN QGPPGPKGAK

    GEVGPPGPPG QFPFDFLQLE AEMKGEKGDR GDAGQKGERG EPGGGGFFGS

    SLPGPPGPPG PPGPRGYPGI PGPKGESIRG QPGPPGPQGP PGIGYEGRQG

    PPGPPGPPGP PSFPGPHRQT ISVPGPPGPP GPPGPPGTMG ASSGVRLWAT

    RQAMLGQVHE VPEGWLIFVA EQEELYVRVQ NGFRKVQLEA RTPLPRGTDN

    EVAALQPPVV QLHDSNPYPR REHPHPTARP WRADDILASP PRLPEPQPYP

    GAPHHSSYVH LRPARPTSPP AHSHRDFQPV LHLVALNSPL SGGMRGIRGA

    DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL

    FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR

    LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM

    TASK

  • UCSC uc062awf.1; human. [P39060-3];


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