Human ENDOG ELISA Kit

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  • Alternative name

    Human endo G ELISA Kit;Human endonuclease G ELISA Kit;Human endonuclease G, mitochondrial ELISA Kit;Human mitochondrial endonuclease G ELISA Kit;

  • Catalog
    E013037
  • species
    Human
  • GeneENDOG
  • Other Species Mouse Endog ELISA KitBovine ENDOG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ENDOG. No significant cross-reactivity or interference between human ENDOG and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ENDOG is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman ENDOG ELISA Kit allows for the in vitro quantitative determination of ENDOG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENDOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENDOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENDOG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENDOG bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Endonuclease G, mitochondrial Protein information
  • Uniprot ID NUCG_HUMAN
  • Uniprot AC Q14249; Q5T281; Q9BSP2;
  • UniGene Hs.745161;
  • GeneID 2021
  • KEGG hsa:2021;
  • Human Endonuclease G, mitochondrial Protein SEQUENCE
  • SEQUENCE 297 AA; 32620 MW; 33015E8A619781B7 CRC64;

    MRALRAGLTL ASGAGLGAVV EGWRRRREDA RAAPGLLGRL PVLPVAAAAE

    LPPVPGGPRG PGELAKYGLP GLAQLKSRES YVLCYDPRTR GALWVVEQLR

    PERLRGDGDR RECDFREDDS VHAYHRATNA DYRGSGFDRG HLAAAANHRW

    SQKAMDDTFY LSNVAPQVPH LNQNAWNNLE KYSRSLTRSY QNVYVCTGPL

    FLPRTEADGK SYVKYQVIGK NHVAVPTHFF KVLILEAAGG QIELRTYVMP

    NAPVDEAIPL ERFLVPIESI ERASGLLFVP NILARAGSLK AITAGSK

  • UCSC uc004bwc.4; human.;


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