Human EG-VEGF ELISA Kit

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  • Alternative name

    Prokineticin-1 ELISA KIT; Endocrine-gland-derived vascular endothelial growth factor ELISA KIT; EG-VEGF ELISA KIT; MambakinePROK1 ELISA KIT; EG-VEGF ELISA KIT

  • Catalog
    E013009
  • species
    Human
  • GeneEG-VEGF
  • Standard CurveHuman EG-VEGF ELISA Kit
  • Other Species Human EG VEGF ELISA KitMouse EG-VEGF ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). No significant cross-reactivity or interference between Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.3pg/mL.
  • Intended UseHuman EG-VEGF ELISA Kit allows for the in vitro quantitative determination of EG-VEGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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