Human ESM1 ELISA Kit

Alternative name
Human ESM-1 ELISA Kit;Human endocan ELISA Kit;Human endothelial cell specific molecule 1 ELISA Kit;Human endothelial cell-specific molecule 1 ELISA Kit;
Catalog
E013006
species
Human
GeneESM1
SpecificityThe Human Endocan ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endocan proteins within the range of 31.2 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseHuman ESM1 ELISA Kit allows for the in vitro quantitative determination of ESM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Bioscience Human Endocan ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endocan in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Endocan coated on a 96-well plate. Standards and samples are pipetted into the wells and Endocan present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endocan antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endocan bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
Human Endothelial cell-specific molecule 1 Protein information
Uniprot ID ESM1_HUMAN
Uniprot AC Q9NQ30; B2R4G3; Q15330; Q3V4E3; Q96ES3;
UniGene Hs.129944;
GeneID 11082
KEGG hsa:11082;
Human Endothelial cell-specific molecule 1 Protein SEQUENCE
SEQUENCE 184 AA; 20095 MW; 2AD15EDEC74BC8CE CRC64;
MKSVLLLTTL LVPAHLVAAW SNNYAVDCPQ HCDSSECKSS PRCKRTVLDD
CGCCRVCAAG RGETCYRTVS GMDGMKCGPG LRCQPSNGED PFGEEFGICK
DCPYGTFGMD CRETCNCQSG ICDRGTGKCL KFPFFQYSVT KSSNRFVSLT
EHDMASGDGN IVREEVVKEN AAGSPVMRKW LNPR
UCSC uc003jpk.4; human. [Q9NQ30-1];

Category

Human ESM1 ELISA Kit

Human ESM-1 ELISA Kit;Human endocan ELISA Kit;Human endothelial cell specific molecule 1 ELISA Kit;Human endothelial cell-specific molecule 1 ELISA Kit;