Human ENGASE ELISA Kit

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  • Alternative name

    Human ENGase ELISA Kit;Human endo-beta-N-acetylglucosaminidase ELISA Kit;Human cytosolic endo-beta-N-acetylglucosaminidase ELISA Kit;Human Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase ELISA Kit;Human Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase ELISA Kit;

  • Catalog
    E013004
  • species
    Human
  • GeneENGASE
  • Standard CurveHuman ENGASE ELISA Kit
  • Other Species Mouse Engase ELISA KitChicken ENGASE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Endo Beta-N-Acetylglucosaminidase (ENGASE). No significant cross-reactivity or interference between Endo Beta-N-Acetylglucosaminidase (ENGASE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.126ng/mL.
  • Intended UseHuman ENGASE ELISA Kit allows for the in vitro quantitative determination of ENGASE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endo Beta-N-Acetylglucosaminidase (ENGASE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endo Beta-N-Acetylglucosaminidase (ENGASE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytosolic endo-beta-N-acetylglucosaminidase Protein information
  • Uniprot ID ENASE_HUMAN
  • Uniprot AC Q8NFI3; Q659F0; Q8TB86; Q9H6U4;
  • UniGene Hs.29288;
  • GeneID 64772
  • KEGG hsa:64772;
  • Human Cytosolic endo-beta-N-acetylglucosaminidase Protein SEQUENCE
  • SEQUENCE 743 AA; 83987 MW; 384DA533AE549FC5 CRC64;

    MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI

    KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL

    EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY

    AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR

    LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP

    FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF

    FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL

    IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF

    VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR

    THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK

    LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA

    RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR

    LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH

    WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL

    LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA

  • UCSC uc002jwv.5; human. [Q8NFI3-1];



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