Human EVL ELISA Kit

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  • Alternative name

    Human ena/vasodilator-stimulated phosphoprotein-like ELISA Kit;Human RNB6 ELISA Kit;Human Enah/Vasp-like ELISA Kit;Human ena/VASP-like protein ELISA Kit;

  • Catalog
    E012999
  • species
    Human
  • GeneEVL
  • Standard CurveHuman EVL ELISA Kit
  • Other Species Mouse Evl ELISA KitRat Evl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Enah/Vasp Like Protein (EVL). No significant cross-reactivity or interference between Enah/Vasp Like Protein (EVL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman EVL ELISA Kit allows for the in vitro quantitative determination of EVL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enah/Vasp Like Protein (EVL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enah/Vasp Like Protein (EVL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enah/Vasp Like Protein (EVL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enah/Vasp Like Protein (EVL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ena/VASP-like protein Protein information
  • Uniprot ID EVL_HUMAN
  • Uniprot AC Q9UI08; A8K105; B7Z3I5; O95884; Q7Z522; Q8TBV1; Q9UF25; Q9UIC2;
  • UniGene Hs.125867;
  • GeneID 51466
  • KEGG hsa:51466;
  • Human Ena/VASP-like protein Protein SEQUENCE
  • SEQUENCE 416 AA; 44620 MW; AD5B67458755D659 CRC64;

    MSEQSICQAR ASVMVYDDTS KKWVPIKPGQ QGFSRINIYH NTASNTFRVV

    GVKLQDQQVV INYSIVKGLK YNQATPTFHQ WRDARQVYGL NFASKEEATT

    FSNAMLFALN IMNSQEGGPS SQRQVQNGPS PDEMDIQRRQ VMEQHQQQRQ

    ESLERRTSAT GPILPPGHPS SAASAPVSCS GPPPPPPPPV PPPPTGATPP

    PPPPLPAGGA QGSSHDESSM SGLAAAIAGA KLRRVQRPED ASGGSSPSGT

    SKSDANRASS GGGGGGLMEE MNKLLAKRRK AASQSDKPAE KKEDESQMED

    PSTSPSPGTR AASQPPNSSE AGRKPWERSN SVEKPVSSIL SRTPSVAKSP

    EAKSPLQSQP HSRMKPAGSV NDMALDAFDL DRMKQEILEE VVRELHKVKE

    EIIDAIRQEL SGISTT

  • UCSC uc001ygt.4; human. [Q9UI08-1];


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