SpecificityThe microplate is coated with the antigens SS-A (Ro), SS-B (La), Sm, RNP/Sm, Scl-70, Jo- 1. All antigen preparations are highly purified by affinity chromatography. The ENAcombi test is specific only for autoantibodies directed to these antigens. No cross reactivities have been observed.
Intended UseHuman ENA ELISA Kit allows for the in vitro quantitative determination of ENA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyAutoimmune; Immunology; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: Extractable nuclear antigens (SS-A (Ro), SS-B (La), Sm, RNP/Sm, Scl-70 and Jo-1) are bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyses to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.