Human ETFDH ELISA Kit

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  • Alternative name

    Human ETF-QO ELISA Kit;Human ETF-ubiquinone oxidoreductase ELISA Kit;Human electron-transferring-flavoprotein dehydrogenase ELISA Kit;Human ETF dehydrogenase ELISA Kit;Human ETFQO ELISA Kit;Human MADD ELISA Kit;Human electron transfer flavoprotein dehydrogenase ELISA Kit;Human electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ELISA Kit;

  • Catalog
    E012915
  • species
    Human
  • GeneETFDH
  • Standard CurveHuman ETFDH ELISA Kit
  • Other Species Mouse Etfdh ELISA KitBovine ETFDH ELISA KitPorcine ETFDH ELISA KitRat Etfdh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Electron Transferring Flavoprotein Dehydrogenase (ETFDH). No significant cross-reactivity or interference between Electron Transferring Flavoprotein Dehydrogenase (ETFDH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.072ng/mL.
  • Intended UseHuman ETFDH ELISA Kit allows for the in vitro quantitative determination of ETFDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transferring Flavoprotein Dehydrogenase (ETFDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transferring Flavoprotein Dehydrogenase (ETFDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transferring Flavoprotein Dehydrogenase (ETFDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transferring Flavoprotein Dehydrogenase (ETFDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Protein information
  • Uniprot ID ETFD_HUMAN
  • Uniprot AC Q16134; B4E3R9; J3KND9; Q7Z347;
  • UniGene Hs.155729;
  • GeneID 2110
  • KEGG hsa:2110;
  • Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Protein SEQUENCE
  • SEQUENCE 617 AA; 68495 MW; 099EBA36C59AF3D6 CRC64;

    MLVPLAKLSC LAYQCFHALK IKKNYLPLCA TRWSSTSTVP RITTHYTIYP

    RDKDKRWEGV NMERFAEEAD VVIVGAGPAG LSAAVRLKQL AVAHEKDIRV

    CLVEKAAQIG AHTLSGACLD PGAFKELFPD WKEKGAPLNT PVTEDRFGIL

    TEKYRIPVPI LPGLPMNNHG NYIVRLGHLV SWMGEQAEAL GVEVYPGYAA

    AEVLFHDDGS VKGIATNDVG IQKDGAPKAT FERGLELHAK VTIFAEGCHG

    HLAKQLYKKF DLRANCEPQT YGIGLKELWV IDEKNWKPGR VDHTVGWPLD

    RHTYGGSFLY HLNEGEPLVA LGLVVGLDYQ NPYLSPFREF QRWKHHPSIR

    PTLEGGKRIA YGARALNEGG FQSIPKLTFP GGLLIGCSPG FMNVPKIKGT

    HTAMKSGILA AESIFNQLTS ENLQSKTIGL HVTEYEDNLK NSWVWKELYS

    VRNIRPSCHG VLGVYGGMIY TGIFYWILRG MEPWTLKHKG SDFERLKPAK

    DCTPIEYPKP DGQISFDLLS SVALSGTNHE HDQPAHLTLR DDSIPVNRNL

    SIYDGPEQRF CPAGVYEFVP VEQGDGFRLQ INAQNCVHCK TCDIKDPSQN

    INWVVPEGGG GPAYNGM

  • UCSC uc003iqb.5; human. [Q16134-1];


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