Human EMILIN1 ELISA Kit

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  • Alternative name

    Human elastin microfibril interface-located protein 1 ELISA Kit;Human elastin microfibril interfacer 1 ELISA Kit;Human EMI ELISA Kit;Human EMILIN ELISA Kit;Human gp115 ELISA Kit;Human EMILIN-1 ELISA Kit;

  • Catalog
    E012890
  • species
    Human
  • GeneEMILIN1
  • Standard CurveHuman EMILIN1 ELISA Kit
  • Other Species Mouse Emilin1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 1 (EMILIN1). No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 1 (EMILIN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.64ng/mL.
  • Intended UseHuman EMILIN1 ELISA Kit allows for the in vitro quantitative determination of EMILIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 1 (EMILIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastin Microfibril Interface Located Protein 1 (EMILIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human EMILIN-1 Protein information
  • Uniprot ID EMIL1_HUMAN
  • Uniprot AC Q9Y6C2; A5PL03; H0Y7A0; Q53SY9; Q96G58; Q96IH6; Q9UG76;
  • UniGene Hs.63348;
  • GeneID 11117
  • KEGG hsa:11117;
  • Human EMILIN-1 Protein SEQUENCE
  • SEQUENCE 1016 AA; 106667 MW; A447F74E17DBCBA8 CRC64;

    MAPRTLWSCY LCCLLTAAAG AASYPPRGFS LYTGSSGALS PGGPQAQIAP

    RPASRHRNWC AYVVTRTVSC VLEDGVETYV KYQPCAWGQP QCPQSIMYRR

    FLRPRYRVAY KTVTDMEWRC CQGYGGDDCA ESPAPALGPA SSTPRPLAQP

    ARPNLSGSSA GSPLSGLGGE GPGESEKVQQ LEEQVQSLTK ELQGLRGVLQ

    GLSGRLAEDV QRAVETAFNG RQQPADAAAR PGVHETLNEI QHQLQLLDTR

    VSTHDQELGH LNNHHGGSSS SGGSRAPAPA SAPPGPSEEL LRQLEQRLQE

    SCSVCLAGLD GFRRQQQEDR ERLRAMEKLL ASVEERQRHL AGLAVGRRPP

    QECCSPELGR RLAELERRLD VVAGSVTVLS GRRGTELGGA AGQGGHPPGY

    TSLASRLSRL EDRFNSTLGP SEEQEESWPG APGGLSHWLP AARGRLEQLG

    GLLANVSGEL GGRLDLLEEQ VAGAMQACGQ LCSGAPGEQD SQVSEILSAL

    ERRVLDSEGQ LRLVGSGLHT VEAAGEARQA TLEGLQEVVG RLQDRVDAQD

    ETAAEFTLRL NLTAARLGQL EGLLQAHGDE GCGACGGVQE ELGRLRDGVE

    RCSCPLLPPR GPGAGPGVGG PSRGPLDGFS VFGGSSGSAL QALQGELSEV

    ILSFSSLNDS LNELQTTVEG QGADLADLGA TKDRIISEIN RLQQEATEHA

    TESEERFRGL EEGQAQAGQC PSLEGRLGRL EGVCERLDTV AGGLQGLREG

    LSRHVAGLWA GLRETNTTSQ MQAALLEKLV GGQAGLGRRL GALNSSLQLL

    EDRLHQLSLK DLTGPAGEAG PPGPPGLQGP PGPAGPPGSP GKDGQEGPIG

    PPGPQGEQGV EGAPAAPVPQ VAFSAALSLP RSEPGTVPFD RVLLNDGGYY

    DPETGVFTAP LAGRYLLSAV LTGHRHEKVE AVLSRSNQGV ARVDSGGYEP

    EGLENKPVAE SQPSPGTLGV FSLILPLQAG DTVCVDLVMG QLAHSEEPLT

    IFSGALLYGD PELEHA

  • UCSC uc002rii.5; human. [Q9Y6C2-1];


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