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  • Alternative name

    Chymotrypsin-like elastase family member 3A ELISA KIT; Elastase IIIA ELISA KIT; Elastase-3A ELISA KIT; Protease ECELA3A ELISA KIT; ELA3 ELISA KIT; ELA3A ELISA KIT

  • Catalog
  • species
  • GeneELA3A
  • Standard CurveHuman ELA3A ELISA Kit
  • Other Species Mouse ELA3A ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Elastase 3A (ELA3A). No significant cross-reactivity or interference between Elastase 3A (ELA3A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 13.4pg/mL.
  • Intended UseHuman ELA3A ELISA Kit allows for the in vitro quantitative determination of ELA3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3A (ELA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3A (ELA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3A (ELA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3A (ELA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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