Human EGR4 ELISA Kit

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  • Alternative name

    Human EGR-4 ELISA Kit;Human AT133 ELISA Kit;Human NGFI-C ELISA Kit;Human NGFIC ELISA Kit;Human PAT133 ELISA Kit;Human early growth response 4 ELISA Kit;Human early growth response protein 4 ELISA Kit;

  • Catalog
    E012837
  • species
    Human
  • GeneEGR4
  • Other Species Mouse Egr4 ELISA KitBovine EGR4 ELISA KitRat Egr4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human EGR4. No significant cross-reactivity or interference between Human EGR4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human EGR4 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman EGR4 ELISA Kit allows for the in vitro quantitative determination of EGR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EGR4 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGR4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human EGR4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human EGR4. You can calculate the concentration of Human EGR4 in the samples by comparing the OD of the samples to the standard curve.
  • Human Early growth response protein 4 Protein information
  • Uniprot ID EGR4_HUMAN
  • Uniprot AC Q05215; B2RAE3; G3V1T5; Q2Z1P5;
  • UniGene Hs.3052;
  • GeneID 1961
  • KEGG hsa:1961;
  • Human Early growth response protein 4 Protein SEQUENCE
  • SEQUENCE 589 AA; 61623 MW; EFB5A743541A07D7 CRC64;

    MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR

    LPDSFPRGEC PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR

    RRAMLHLSEF SEPDALLVKS TEGCCAEPSA ELPRLPARDA PAATGYPGAG

    DFLSWALNSC GASGDLADSC FLEGPAPTPP PGLSYSGSFF IQAVPEHPHD

    PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL LPGPPDLYSP

    DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA

    VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS

    CPAELPAVPA NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS

    GDGGEFLAST QPQLSPLGLR SAAAADFPKP LVADIPGSSG VAAPPVPPPP

    PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA KAFACPVESC VRSFARSDEL

    NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP FACDVCGRRF

    ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASL

  • UCSC uc010yrj.3; human.;




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