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Human EGFR ELISA Kit

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  • Alternative name

    Human proto-oncogene c-ErbB-1 ELISA Kit;Human receptor tyrosine-protein kinase erbB-1 ELISA Kit;Human ERBB ELISA Kit;Human ERBB1 ELISA Kit;Human HER1 ELISA Kit;Human NISBD2 ELISA Kit;Human PIG61 ELISA Kit;Human mENA ELISA Kit;Human epidermal growth factor receptor ELISA Kit;Human avian erythroblastic leukemia viral (v-erb-b) oncogene homolog ELISA Kit;Human cell growth inhibiting protein 40 ELISA Kit;Human cell proliferation-inducing protein 61 ELISA Kit;Human erb-b2 receptor tyrosine kinase 1 ELISA Kit;
  • Catalog
    E012828
  • species
    Human
  • GeneEGFR
  • SpecificityHER-2/neu p105 is not detected by the EGFr ELISA when tested at levels ten-fold higher than the Level 6 Standard.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe EGFr ELISA will detect 0.25 ng/mL of EGFr analyte in a Sample Diluent matrix. The signal of the 0.25 ng/mL Standard is approximately two times the zero signal.
  • Intended UseHuman EGFR ELISA Kit allows for the in vitro quantitative determination of EGFR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCytokines & Cell Signalling; Oncology; Research Assays; Assay Categories
  • Product Description
    specifical    Principle of the assay: The EGFr ELISA is a sandwich type immunoassay that uses a mouse monoclonal Capture Antibody and an alkaline phosphatase-labeled mouse monoclonal antibody as detector. Both capture and detector reagents specifically recognize the extracellular domain of EGFr. The Capture Antibody recognizes a protein domain on the extracellular portion of EGFr, does not inhibit EGF binding, and does not crossreact with erbB-2 oncoprotein or human blood group A antigen. The Capture Antibody has been immobilized on the interior surface of the microtiter plate wells. To perform the test, an appropriate volume of specimen is incubated in the wells to allow binding of the antigen by the Capture Antibody. The immobilized antigen is then exposed to the alkaline phosphatase-labeled Detector Antibody. Addition of Substrate to the wells allows the catalysis of a chromogen into a colored product, the intensity of which is proportional to the amount of EGFr that is bound to the plate. Standards are provided in the kit that allow accurate, quantitative determinations of EGFr in suitable samples. Using a microtiter plate reader, one can measure simultaneously the absorbance of the colored product in the Standards and sample wells. Correlating the absorbance values of samples with the Standards allows the investigator to determine the levels of EGFr in a sample. Samples may be assigned a quantitative value of EGFr in nanograms per mL (ng/mL) of serum or plasma. For instructions, see the Detailed Protocol and Evaluation of Results sections of this booklet.
  • Human Epidermal growth factor receptor Protein information
  • Uniprot ID EGFR_HUMAN
  • Uniprot AC Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7; Q9UMD8; Q9UMG5;
  • UniGene Hs.488293; Hs.605083; Hs.731652;
  • GeneID 1956
  • KEGG hsa:1956;
  • Human Epidermal growth factor receptor Protein SEQUENCE

  • SEQUENCE 1210 AA; 134277 MW; D8A2A50B4EFB6ED2 CRC64;

    MRPSGTAGAA LLALLAALCP ASRALEEKKV CQGTSNKLTQ LGTFEDHFLS

    LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP

    LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF

    SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW

    GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV

    CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV

    VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS

    INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE

    ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL

    RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK

    ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCN LLEGEPREFV

    ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM

    GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIATGM

    VGALLLLLVV ALGIGLFMRR RHIVRKRTLR RLLQERELVE PLTPSGEAPN

    QALLRILKET EFKKIKVLGS GAFGTVYKGL WIPEGEKVKI PVAIKELREA

    TSPKANKEIL DEAYVMASVD NPHVCRLLGI CLTSTVQLIT QLMPFGCLLD

    YVREHKDNIG SQYLLNWCVQ IAKGMNYLED RRLVHRDLAA RNVLVKTPQH

    VKITDFGLAK LLGAEEKEYH AEGGKVPIKW MALESILHRI YTHQSDVWSY

    GVTVWELMTF GSKPYDGIPA SEISSILEKG ERLPQPPICT IDVYMIMVKC

    WMIDADSRPK FRELIIEFSK MARDPQRYLV IQGDERMHLP SPTDSNFYRA

    LMDEEDMDDV VDADEYLIPQ QGFFSSPSTS RTPLLSSLSA TSNNSTVACI

    DRNGLQSCPI KEDSFLQRYS SDPTGALTED SIDDTFLPVP EYINQSVPKR

    PAGSVQNPVY HNQPLNPAPS RDPHYQDPHS TAVGNPEYLN TVQPTCVNST

    FDSPAHWAQK GSHQISLDNP DYQQDFFPKE AKPNGIFKGS TAENAEYLRV

    APQSSEFIGA

  • UCSC uc003tqh.4; human. [P00533-1];



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