Human EGF ELISA Kit

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  • Alternative name

    Human EGF ELISA Kit;Human Urogastrone ELISA Kit;Human HOMG4 ELISA Kit;Human URG ELISA Kit;Human epidermal growth factor ELISA Kit;Human pro-epidermal growth factor ELISA Kit;Human beta-urogastrone ELISA Kit;

  • Catalog
    E012799
  • species
    Human
  • GeneEGF
  • Standard CurveHuman EGF ELISA Kit
  • Other Species Human HB EGF ELISA KitHuman HB-EGF ELISA KitMouse Egf ELISA KitMouse HB-EGF ELISA KitCanine EGF ELISA KitPorcine EGF ELISA KitRat Egf ELISA Kit
  • SpecificityNatural and recombinant human EGF
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman EGF ELISA Kit allows for the in vitro quantitative determination of EGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human EGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGF has been precoated onto 96-well plates. Standards (E.coli,N971-R1023) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EGF amount of sample captured in plate. Background: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds.1 EGF acts by binding with high affinity to epidermalgrowth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival.2 It also has a profound effect on thedifferentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.3 EGF has strong expression in kidney, salivary gland, cerebrum, and prostate,moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.4
  • Human Epidermal growth factor Protein information
  • Uniprot ID EGF_HUMAN
  • Uniprot AC P01133; B4DRK7; E7EVD2; E9PBF0; Q52LZ6;
  • UniGene Hs.419815;
  • GeneID 1950
  • KEGG hsa:1950;
  • Human Epidermal growth factor Protein SEQUENCE
  • SEQUENCE 1207 AA; 133994 MW; 3C787F1D405CFAF1 CRC64;

    MLLTLIILLP VVSKFSFVSL SAPQHWSCPE GTLAGNGNST CVGPAPFLIF

    SHGNSIFRID TEGTNYEQLV VDAGVSVIMD FHYNEKRIYW VDLERQLLQR

    VFLNGSRQER VCNIEKNVSG MAINWINEEV IWSNQQEGII TVTDMKGNNS

    HILLSALKYP ANVAVDPVER FIFWSSEVAG SLYRADLDGV GVKALLETSE

    KITAVSLDVL DKRLFWIQYN REGSNSLICS CDYDGGSVHI SKHPTQHNLF

    AMSLFGDRIF YSTWKMKTIW IANKHTGKDM VRINLHSSFV PLGELKVVHP

    LAQPKAEDDT WEPEQKLCKL RKGNCSSTVC GQDLQSHLCM CAEGYALSRD

    RKYCEDVNEC AFWNHGCTLG CKNTPGSYYC TCPVGFVLLP DGKRCHQLVS

    CPRNVSECSH DCVLTSEGPL CFCPEGSVLE RDGKTCSGCS SPDNGGCSQL

    CVPLSPVSWE CDCFPGYDLQ LDEKSCAASG PQPFLLFANS QDIRHMHFDG

    TDYGTLLSQQ MGMVYALDHD PVENKIYFAH TALKWIERAN MDGSQRERLI

    EEGVDVPEGL AVDWIGRRFY WTDRGKSLIG RSDLNGKRSK IITKENISQP

    RGIAVHPMAK RLFWTDTGIN PRIESSSLQG LGRLVIASSD LIWPSGITID

    FLTDKLYWCD AKQSVIEMAN LDGSKRRRLT QNDVGHPFAV AVFEDYVWFS

    DWAMPSVMRV NKRTGKDRVR LQGSMLKPSS LVVVHPLAKP GADPCLYQNG

    GCEHICKKRL GTAWCSCREG FMKASDGKTC LALDGHQLLA GGEVDLKNQV

    TPLDILSKTR VSEDNITESQ HMLVAEIMVS DQDDCAPVGC SMYARCISEG

    EDATCQCLKG FAGDGKLCSD IDECEMGVPV CPPASSKCIN TEGGYVCRCS

    EGYQGDGIHC LDIDECQLGE HSCGENASCT NTEGGYTCMC AGRLSEPGLI

    CPDSTPPPHL REDDHHYSVR NSDSECPLSH DGYCLHDGVC MYIEALDKYA

    CNCVVGYIGE RCQYRDLKWW ELRHAGHGQQ QKVIVVAVCV VVLVMLLLLS

    LWGAHYYRTQ KLLSKNPKNP YEESSRDVRS RRPADTEDGM SSCPQPWFVV

    IKEHQDLKNG GQPVAGEDGQ AADGSMQPTS WRQEPQLCGM GTEQGCWIPV

    SSDKGSCPQV MERSFHMPSY GTQTLEGGVE KPHSLLSANP LWQQRALDPP

    HQMELTQ

  • UCSC uc003hzy.5; human. [P01133-1];



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