Human EDA ELISA Kit

Alternative name
Human Ectodermal dysplasia protein ELISA Kit;Human EDA protein ELISA Kit;Human ED1 ELISA Kit;Human EDA2 ELISA Kit;Human ECTD1 ELISA Kit;Human ED1-A1 ELISA Kit;Human ED1-A2 ELISA Kit;Human EDA-A1 ELISA Kit;Human EDA-A2 ELISA Kit;Human EDA1 ELISA Kit;Human HED ELISA Kit;Human HED1 ELISA Kit;Human ODT1 ELISA Kit;Human STHAGX1 ELISA Kit;Human TNLG7C ELISA Kit;Human XHED ELISA Kit;Human XLHED ELISA Kit;Human ectodysplasin A ELISA Kit;Human ectodysplasin-A ELISA Kit;Human X-linked anhidroitic ectodermal dysplasia protein ELISA Kit;Human oligodontia 1 ELISA Kit;Human tumor necrosis factor ligand 7C ELISA Kit;
Catalog
E012735
species
Human
GeneEDA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman EDA ELISA Kit allows for the in vitro quantitative determination of EDA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human EDA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EDA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EDA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman EDA can be calculated.
Human Ectodysplasin-A, secreted form Protein information
Uniprot ID EDA_HUMAN
Uniprot AC Q9UP77; Q9Y6L0; Q9Y6L1; Q9Y6L2; Q9Y6L3; Q9Y6L4;
UniGene Hs.105407;
GeneID 1896
KEGG hsa:1896;
Human Ectodysplasin-A, secreted form Protein SEQUENCE
SEQUENCE 391 AA; 41294 MW; 15DB3F5053293CBA CRC64;
MGYPEVERRE LLPAAAPRER GSQGCGCGGA PARAGEGNSC LLFLGFFGLS
LALHLLTLCC YLELRSELRR ERGAESRLGG SGTPGTSGTL SSLGGLDPDS
PITSHLGQPS PKQQPLEPGE AALHSDSQDG HQMALLNFFF PDEKPYSEEE
SRRVRRNKRS KSNEGADGPV KNKKKGKKAG PPGPNGPPGP PGPPGPQGPP
GIPGIPGIPG TTVMGPPGPP GPPGPQGPPG LQGPSGAADK AGTRENQPAV
VHLQGQGSAI QVKNDLSGGV LNDWSRITMN PKVFKLHPRS GELEVLVDGT
YFIYSQVEVY YINFTDFASY EVVVDEKPFL QCTRSIETGK TNYNTCYTAG
VCLLKARQKI AVKMVHADIS INMSKHTTFF GAIRLGEAPA S
UCSC uc004dxm.2; human. [Q92838-1];

Category

Human EDA ELISA Kit