Human ECE1 ELISA Kit

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  • Alternative name

    Human ECE-1 ELISA Kit;Human ECE ELISA Kit;Human endothelin converting enzyme 1 ELISA Kit;Human endothelin-converting enzyme 1 ELISA Kit;

  • Catalog
    E012707
  • species
    Human
  • GeneECE1
  • Other Species Mouse Ece1 ELISA KitBovine ECE1 ELISA KitGuinea Pig ECE1 ELISA KitRat Ece1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ECE1. No significant cross-reactivity or interference between Human ECE1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ECE1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity18.75pg/mL
  • Intended UseHuman ECE1 ELISA Kit allows for the in vitro quantitative determination of ECE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ECE1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECE1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ECE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ECE1. You can calculate the concentration of Human ECE1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Endothelin-converting enzyme 1 Protein information
  • Uniprot ID ECE1_HUMAN
  • Uniprot AC Q5THM7; Q5THM8; Q9UJQ6; Q9UPF4; Q9UPM4; Q9Y501;
  • UniGene Hs.195080;
  • GeneID 1889
  • KEGG hsa:1889;
  • Human Endothelin-converting enzyme 1 Protein SEQUENCE
  • SEQUENCE 770 AA; 87164 MW; DD88A59748B22F80 CRC64;

    MRGVWPPPVS ALLSALGMST YKRATLDEED LVDSLSEGDA YPNGLQVNFH

    SPRSGQRCWA ARTQVEKRLV VLVVLLAAGL VACLAALGIQ YQTRSPSVCL

    SEACVSVTSS ILSSMDPTVD PCHDFFSYAC GGWIKANPVP DGHSRWGTFS

    NLWEHNQAII KHLLENSTAS VSEAERKAQV YYRACMNETR IEELRAKPLM

    ELIERLGGWN ITGPWAKDNF QDTLQVVTAH YRTSPFFSVY VSADSKNSNS

    NVIQVDQSGL GLPSRDYYLN KTENEKVLTG YLNYMVQLGK LLGGGDEEAI

    RPQMQQILDF ETALANITIP QEKRRDEELI YHKVTAAELQ TLAPAINWLP

    FLNTIFYPVE INESEPIVVY DKEYLEQIST LINTTDRCLL NNYMIWNLVR

    KTSSFLDQRF QDADEKFMEV MYGTKKTCLP RWKFCVSDTE NNLGFALGPM

    FVKATFAEDS KSIATEIILE IKKAFEESLS TLKWMDEETR KSAKEKADAI

    YNMIGYPNFI MDPKELDKVF NDYTAVPDLY FENAMRFFNF SWRVTADQLR

    KAPNRDQWSM TPPMVNAYYS PTKNEIVFPA GILQAPFYTR SSPKALNFGG

    IGVVVGHELT HAFDDQGREY DKDGNLRPWW KNSSVEAFKR QTECMVEQYS

    NYSVNGEPVN GRHTLGENIA DNGGLKAAYR AYQNWVKKNG AEHSLPTLGL

    TNNQLFFLGF AQVWCSVRTP ESSHEGLITD PHSPSRFRVI GSLSNSKEFS

    EHFRCPPGSP MNPPHKCEVW

  • UCSC uc001bei.3; human. [P42892-1];


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