Human EGR1 ELISA Kit

Alternative name
Human EGR-1 ELISA Kit;Human AT225 ELISA Kit;Human nerve growth factor-induced protein A ELISA Kit;Human NGFI-A ELISA Kit;Human transcription factor ETR103 ELISA Kit;Human transcription factor Zif268 ELISA Kit;Human zinc finger protein 225 ELISA Kit;Human zinc finger protein Krox-24 ELISA Kit;Human KROX24 ELISA Kit;Human ZNF225 ELISA Kit;Human G0S30 ELISA Kit;Human KROX-24 ELISA Kit;Human TIS8 ELISA Kit;Human ZIF-268 ELISA Kit;Human early growth response 1 ELISA Kit;Human early growth response protein 1 ELISA Kit;
Catalog
E012684
species
Human
GeneEGR1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human EGR1. No significant cross-reactivity or interference between human EGR1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human EGR1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman EGR1 ELISA Kit allows for the in vitro quantitative determination of EGR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Early growth response protein 1 Protein information
Uniprot ID EGR1_HUMAN
Uniprot AC P18146;
UniGene Hs.326035; Hs.708393;
GeneID 1958
KEGG hsa:1958;
Human Early growth response protein 1 Protein SEQUENCE
SEQUENCE 543 AA; 57507 MW; 768CE670D9743E4E CRC64;
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
AAGAPEGSGS NSSSSSSGGG GGGGGGSNSS SSSSTFNPQA DTGEQPYEHL
TAESFPDISL NNEKVLVETS YPSQTTRLPP ITYTGRFSLE PAPNSGNTLW
PEPLFSLVSG LVSMTNPPAS SSSAPSPAAS SASASQSPPL SCAVPSNDSS
PIYSAAPTFP TPNTDIFPEP QSQAFPGSAG TALQYPPPAY PAAKGGFQVP
MIPDYLFPQQ QGDLGLGTPD QKPFQGLESR TQQPSLTPLS TIKAFATQSG
SQDLKALNTS YQSQLIKPSR MRKYPNRPSK TPPHERPYAC PVESCDRRFS
RSDELTRHIR IHTGQKPFQC RICMRNFSRS DHLTTHIRTH TGEKPFACDI
CGRKFARSDE RKRHTKIHLR QKDKKADKSV VASSATSSLS SYPSPVATSY
PSPVTTSYPS PATTSYPSPV PTSFSSPGSS TYPSPVHSGF PSPSVATTYS
SVPPAFPAQV SSFPSSAVTN SFSASTGLSD MTATFSPRTI EIC

Category

Human EGR1 ELISA Kit