Human EEA1 ELISA Kit

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  • Alternative name

    Human endosome-associated protein p162 ELISA Kit;Human zinc finger FYVE domain-containing protein 2 ELISA Kit;Human ZFYVE2 ELISA Kit;Human MST105 ELISA Kit;Human MSTP105 ELISA Kit;Human early endosome antigen 1 ELISA Kit;Human early endosome antigen 1, 162kD ELISA Kit;Human early endosome-associated protein ELISA Kit;

  • Catalog
    E012683
  • species
    Human
  • GeneEEA1
  • Standard CurveHuman EEA1 ELISA Kit
  • Other Species Mouse Eea1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Early Endosome Antigen 1 (EEA1). No significant cross-reactivity or interference between Early Endosome Antigen 1 (EEA1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman EEA1 ELISA Kit allows for the in vitro quantitative determination of EEA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Endosome Antigen 1 (EEA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Early Endosome Antigen 1 (EEA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Endosome Antigen 1 (EEA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Early Endosome Antigen 1 (EEA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Early endosome antigen 1 Protein information
  • Uniprot ID EEA1_HUMAN
  • Uniprot AC Q15075; Q14221;
  • UniGene Hs.567367;
  • GeneID 8411
  • KEGG hsa:8411;
  • Human Early endosome antigen 1 Protein SEQUENCE
  • SEQUENCE 1411 AA; 162466 MW; 51BA418F561E3411 CRC64;

    MLRRILQRTP GRVGSQGSDL DSSATPINTV DVNNESSSEG FICPQCMKSL

    GSADELFKHY EAVHDAGNDS GHGGESNLAL KRDDVTLLRQ EVQDLQASLK

    EEKWYSEELK KELEKYQGLQ QQEAKPDGLV TDSSAELQSL EQQLEEAQTE

    NFNIKQMKDL FEQKAAQLAT EIADIKSKYD EERSLREAAE QKVTRLTEEL

    NKEATVIQDL KTELLQRPGI EDVAVLKKEL VQVQTLMDNM TLERERESEK

    LKDECKKLQS QYASSEATIS QLRSELAKGP QEVAVYVQEL QKLKSSVNEL

    TQKNQTLTEN LLKKEQDYTK LEEKHNEESV SKKNIQATLH QKDLDCQQLQ

    SRLSASETSL HRIHVELSEK GEATQKLKEE LSEVETKYQH LKAEFKQLQQ

    QREEKEQHGL QLQSEINQLH SKLLETERQL GEAHGRLKEQ RQLSSEKLMD

    KEQQVADLQL KLSRLEEQLK EKVTNSTELQ HQLDKTKQQH QEQQALQQST

    TAKLREAQND LEQVLRQIGD KDQKIQNLEA LLQKSKENIS LLEKEREDLY

    AKIQAGEGET AVLNQLQEKN HTLQEQVTQL TEKLKNQSES HKQAQENLHD

    QVQEQKAHLR AAQDRVLSLE TSVNELNSQL NESKEKVSQL DIQIKAKTEL

    LLSAEAAKTA QRADLQNHLD TAQNALQDKQ QELNKITTQL DQVTAKLQDK

    QEHCSQLESH LKEYKEKYLS LEQKTEELEG QIKKLEADSL EVKASKEQAL

    QDLQQQRQLN TDLELRATEL SKQLEMEKEI VSSTRLDLQK KSEALESIKQ

    KLTKQEEEKK ILKQDFETLS QETKIQHEEL NNRIQTTVTE LQKVKMEKEA

    LMTELSTVKD KLSKVSDSLK NSKSEFEKEN QKGKAAILDL EKTCKELKHQ

    LQVQMENTLK EQKELKKSLE KEKEASHQLK LELNSMQEQL IQAQNTLKQN

    EKEEQQLQGN INELKQSSEQ KKKQIEALQG ELKIAVLQKT ELENKLQQQL

    TQAAQELAAE KEKISVLQNN YEKSQETFKQ LQSDFYGRES ELLATRQDLK

    SVEEKLSLAQ EDLISNRNQI GNQNKLIQEL KTAKATLEQD SAKKEQQLQE

    RCKALQDIQK EKSLKEKELV NEKSKLAEIE EIKCRQEKEI TKLNEELKSH

    KLESIKEITN LKDAKQLLIQ QKLELQGKAD SLKAAVEQEK RNQQILKDQV

    KKEEEELKKE FIEKEAKLHS EIKEKEVGMK KHEENEAKLT MQITALNENL

    GTVKKEWQSS QRRVSELEKQ TDDLRGEIAV LEATVQNNQD ERRALLERCL

    KGEGEIEKLQ TKVLELQRKL DNTTAAVQEL GRENQSLQIK HTQALNRKWA

    EDNEVQNCMA CGKGFSVTVR RHHCRQCGNI FCAECSAKNA LTPSSKKPVR

    VCDACFNDLQ G

  • UCSC uc001tck.4; human.;


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