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  • Alternative name

    E3 ubiquitin-protein ligase UBR5 ELISA KIT; E3 ubiquitin-protein ligase, HECT domain-containing 1 ELISA KIT; Hyperplastic discs protein homolog ELISA KIT; hHYD ELISA KIT; Progestin-induced proteinUBR5 ELISA KIT; EDD ELISA KIT; EDD1 ELISA KIT; HYD ELISA KIT; KIAA0896 ELISA KIT; hHYD ELISA KIT

  • Catalog
  • species
  • GeneE3/UBPL
  • SpecificityNo significant cross-reactivity or interference between Human E3/UBPL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/ml.
  • Intended UseHuman E3/UBPL ELISA Kit allows for the in vitro quantitative determination of E3/UBPL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated E3/UBPL concentrations in Human serum, plasma and other body fluids. Using Purified Human E3/UBPL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add E3/UBPL and E3/UBPL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E3/UBPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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