Human E2F2 ELISA Kit

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  • Alternative name

    Human E2F-2 ELISA Kit;Human E2F transcription factor 2 ELISA Kit;Human transcription factor E2F2 ELISA Kit;

  • Catalog
    E012626
  • species
    Human
  • GeneE2F2
  • Standard CurveHuman E2F2 ELISA Kit
  • Other Species Mouse E2f2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 2 (E2F2). No significant cross-reactivity or interference between E2F Transcription Factor 2 (E2F2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman E2F2 ELISA Kit allows for the in vitro quantitative determination of E2F2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 2 (E2F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 2 (E2F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 2 (E2F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 2 (E2F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Transcription factor E2F2 Protein information
  • Uniprot ID E2F2_HUMAN
  • Uniprot AC Q14209; B2R9W1; Q7Z6H1;
  • UniGene Hs.194333;
  • GeneID 1870
  • KEGG hsa:1870;
  • Human Transcription factor E2F2 Protein SEQUENCE
  • SEQUENCE 437 AA; 47506 MW; 60541F4235507005 CRC64;

    MLQGPRALAS AAGQTPKVVP AMSPTELWPS GLSSPQLCPA TATYYTPLYP

    QTAPPAAAPG TCLDATPHGP EGQVVRCLPA GRLPAKRKLD LEGIGRPVVP

    EFPTPKGKCI RVDGLPSPKT PKSPGEKTRY DTSLGLLTKK FIYLLSESED

    GVLDLNWAAE VLDVQKRRIY DITNVLEGIQ LIRKKAKNNI QWVGRGMFED

    PTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE DKANKRLAYV

    TYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEV

    YLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVP

    APAPTPQQAP PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPL

    ISFSPSLDQD DYLWGLEAGE GISDLFDSYD LGDLLIN

  • UCSC uc001bhe.3; human.;




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