Human E2F2 ELISA Kit

Alternative name
Human E2F-2 ELISA Kit;Human E2F transcription factor 2 ELISA Kit;Human transcription factor E2F2 ELISA Kit;
Catalog
E012626
species
Human
GeneE2F2
Standard Curve
Other Species Mouse E2f2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 2 (E2F2). No significant cross-reactivity or interference between E2F Transcription Factor 2 (E2F2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman E2F2 ELISA Kit allows for the in vitro quantitative determination of E2F2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 2 (E2F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 2 (E2F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 2 (E2F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 2 (E2F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Transcription factor E2F2 Protein information
Uniprot ID E2F2_HUMAN
Uniprot AC Q14209; B2R9W1; Q7Z6H1;
UniGene Hs.194333;
GeneID 1870
KEGG hsa:1870;
Human Transcription factor E2F2 Protein SEQUENCE
SEQUENCE 437 AA; 47506 MW; 60541F4235507005 CRC64;
MLQGPRALAS AAGQTPKVVP AMSPTELWPS GLSSPQLCPA TATYYTPLYP
QTAPPAAAPG TCLDATPHGP EGQVVRCLPA GRLPAKRKLD LEGIGRPVVP
EFPTPKGKCI RVDGLPSPKT PKSPGEKTRY DTSLGLLTKK FIYLLSESED
GVLDLNWAAE VLDVQKRRIY DITNVLEGIQ LIRKKAKNNI QWVGRGMFED
PTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE DKANKRLAYV
TYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEV
YLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVP
APAPTPQQAP PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPL
ISFSPSLDQD DYLWGLEAGE GISDLFDSYD LGDLLIN
UCSC uc001bhe.3; human.;

Category

Human E2F2 ELISA Kit

Human E2F-2 ELISA Kit;Human E2F transcription factor 2 ELISA Kit;Human transcription factor E2F2 ELISA Kit;