Human ADH1B ELISA Kit

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  • Alternative name

    Human alcohol dehydrogenase subunit beta ELISA Kit;Human ADH2 ELISA Kit;Human HEL-S-117 ELISA Kit;Human alcohol dehydrogenase 1B (class I), beta polypeptide ELISA Kit;Human alcohol dehydrogenase 1B ELISA Kit;Human ADH, beta subunit ELISA Kit;Human alcohol dehydrogenase 2 (class I), beta polypeptide ELISA Kit;Human aldehyde reductase ELISA Kit;Human epididymis secretory protein Li 117 ELISA Kit;

  • Catalog
    E001261
  • species
    Human
  • GeneADH1B
  • Standard CurveHuman ADH1B ELISA Kit
  • Other Species Mouse ADH1B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADH1B. No significant cross-reactivity or interference between ADH1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADH1B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ADH1B ELISA Kit allows for the in vitro quantitative determination of ADH1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: ADH1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADH1B antibody and an ADH1B-HRP conjugate. The assay sample and buffer are incubated together with ADH1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH1B concentration since ADH1B from samples and ADH1B-HRP conjugate compete for the anti-ADH1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH1B from the sample, fewer sites are left to bind ADH1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH1B concentration in each sample is interpolated from this standard curve.
  • Human Alcohol dehydrogenase 1B Protein information
  • Uniprot ID ADH1B_HUMAN
  • Uniprot AC P00325; A8MYN5; B4DRS9; B4DVC3; Q13711; Q4ZGI9; Q96KI7;
  • UniGene Hs.4;
  • GeneID 125
  • KEGG hsa:125;
  • Human Alcohol dehydrogenase 1B Protein SEQUENCE
  • SEQUENCE 375 AA; 39855 MW; 6962B9A0F967673B CRC64;

    MSTAGKVIKC KAAVLWEVKK PFSIEDVEVA PPKAYEVRIK MVAVGICRTD

    DHVVSGNLVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK

    CRVCKNPESN YCLKNDLGNP RGTLQDGTRR FTCRGKPIHH FLGTSTFSQY

    TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG

    LGGVGLSAVM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP

    IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPASQ

    NLSINPMLLL TGRTWKGAVY GGFKSKEGIP KLVADFMAKK FSLDALITHV

    LPFEKINEGF DLLHSGKSIR TVLTF

  • UCSC uc011cei.3; human. [P00325-1];


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