Human E Cad ELISA Kit

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  • Alternative name

    Cadherin-1 ELISA KIT; CAM 120/80 ELISA KIT; Epithelial cadherin ELISA KIT; E-cadherin ELISA KIT; Uvomorulin ELISA KIT; CD_antigen: CD324Cleaved into the following 3 chains:E-Cad/CTF1 ELISA KIT; E-Cad/CTF2 ELISA KIT; E-Cad/CTF3CDH1 ELISA KIT; CDHE ELISA KIT; UVO ELISA KIT; E-cadherin ELISA KIT

  • Catalog
    E012601
  • species
    Human
  • GeneE Cad
  • Standard CurveHuman E Cad ELISA Kit
  • Other Species Human E-Cad ELISA KitMouse E-Cad ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman E Cad ELISA Kit allows for the in vitro quantitative determination of E Cad , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This ECN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ECN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ECN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ECN. Standards or samples are then added to the microtiter plate wells and ECN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ECN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ECN are added to each well to "sandwich" the ECN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ECN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECN concentration in each sample is interpolated from this standard curve.



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