Human DTNB ELISA Kit

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  • Alternative name

    Human DTN-B ELISA Kit;Human beta-dystrobrevin ELISA Kit;Human dystrobrevin beta ELISA Kit;

  • Catalog
    E012576
  • species
    Human
  • GeneDTNB
  • Standard CurveHuman DTNB ELISA Kit
  • Other Species Mouse Dtnb ELISA KitRat Dtnb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dystrobrevin Beta (DTNb). No significant cross-reactivity or interference between Dystrobrevin Beta (DTNb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman DTNB ELISA Kit allows for the in vitro quantitative determination of DTNB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrobrevin Beta (DTNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrobrevin Beta (DTNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrobrevin Beta (DTNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrobrevin Beta (DTNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dystrobrevin beta Protein information
  • Uniprot ID DTNB_HUMAN
  • Uniprot AC Q96AW0; Q9UE14; Q9UE15; Q9UE16;
  • UniGene Hs.307720;
  • GeneID 1838
  • KEGG hsa:1838;
  • Human Dystrobrevin beta Protein SEQUENCE
  • SEQUENCE 627 AA; 71356 MW; 6927EC0BD86D375C CRC64;

    MIEESGNKRK TMAEKRQLFI EMRAQNFDVI RLSTYRTACK LRFVQKRCNL

    HLVDIWNMIE AFRDNGLNTL DHTTEISVSR LETVISSIYY QLNKRLPSTH

    QISVEQSISL LLNFMIAAYD SEGRGKLTVF SVKAMLATMC GGKMLDKLRY

    VFSQMSDSNG LMIFSKFDQF LKEVLKLPTA VFEGPSFGYT EHSVRTCFPQ

    QRKIMLNMFL DTMMADPPPQ CLVWLPLMHR LAHVENVFHP VECSYCRCES

    MMGFRYRCQQ CHNYQLCQNC FWRGHAGGPH SNQHQMKEHS SWKSPAKKLS

    HAISKSLGCV PTREPPHPVF PEQPEKPLDL AHIVPPRPLT NMNDTMVSHM

    SSGVPTPTKR LQYSQDIPSH LADEHALIAS YVARLQHCAR VLDSPSRLDE

    EHRLIARYAA RLAAEAGNVT RPPTDLSFNF DANKQQRQLI AELENKNREI

    LQEIQRLRLE HEQASQPTPE KAQQNPTLLA ELRLLRQRKD ELEQRMSALQ

    ESRRELMVQL EELMKLLKEE EQKQAAQATG SPHTSPTHGG GRPMPMPVRS

    TSAGSTPTHC PQDSLSGVGG DVQEAFAQGT RRNLRNDLLV AADSITNTMS

    SLVKELHSAE EGAEEEEEKM QNGKDRG

  • UCSC uc002rgh.5; human. [O60941-1];


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