Human DTNB ELISA Kit

Alternative name
Human DTN-B ELISA Kit;Human beta-dystrobrevin ELISA Kit;Human dystrobrevin beta ELISA Kit;
Catalog
E012576
species
Human
GeneDTNB
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dystrobrevin Beta (DTNb). No significant cross-reactivity or interference between Dystrobrevin Beta (DTNb) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman DTNB ELISA Kit allows for the in vitro quantitative determination of DTNB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrobrevin Beta (DTNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrobrevin Beta (DTNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrobrevin Beta (DTNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrobrevin Beta (DTNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dystrobrevin beta Protein information
Uniprot ID DTNB_HUMAN
Uniprot AC Q96AW0; Q9UE14; Q9UE15; Q9UE16;
UniGene Hs.307720;
GeneID 1838
KEGG hsa:1838;
Human Dystrobrevin beta Protein SEQUENCE
SEQUENCE 627 AA; 71356 MW; 6927EC0BD86D375C CRC64;
MIEESGNKRK TMAEKRQLFI EMRAQNFDVI RLSTYRTACK LRFVQKRCNL
HLVDIWNMIE AFRDNGLNTL DHTTEISVSR LETVISSIYY QLNKRLPSTH
QISVEQSISL LLNFMIAAYD SEGRGKLTVF SVKAMLATMC GGKMLDKLRY
VFSQMSDSNG LMIFSKFDQF LKEVLKLPTA VFEGPSFGYT EHSVRTCFPQ
QRKIMLNMFL DTMMADPPPQ CLVWLPLMHR LAHVENVFHP VECSYCRCES
MMGFRYRCQQ CHNYQLCQNC FWRGHAGGPH SNQHQMKEHS SWKSPAKKLS
HAISKSLGCV PTREPPHPVF PEQPEKPLDL AHIVPPRPLT NMNDTMVSHM
SSGVPTPTKR LQYSQDIPSH LADEHALIAS YVARLQHCAR VLDSPSRLDE
EHRLIARYAA RLAAEAGNVT RPPTDLSFNF DANKQQRQLI AELENKNREI
LQEIQRLRLE HEQASQPTPE KAQQNPTLLA ELRLLRQRKD ELEQRMSALQ
ESRRELMVQL EELMKLLKEE EQKQAAQATG SPHTSPTHGG GRPMPMPVRS
TSAGSTPTHC PQDSLSGVGG DVQEAFAQGT RRNLRNDLLV AADSITNTMS
SLVKELHSAE EGAEEEEEKM QNGKDRG
UCSC uc002rgh.5; human. [O60941-1];

Category

Human DTNB ELISA Kit

Human DTN-B ELISA Kit;Human beta-dystrobrevin ELISA Kit;Human dystrobrevin beta ELISA Kit;