Human ADH ELISA Kit

Contact Us
  • Alternative name

    Alcohol dehydrogenase ELISA KIT; Adh ELISA KIT; CG3481 ELISA KIT

  • Catalog
    E001254
  • species
    Human
  • GeneADH
  • Standard CurveHuman ADH ELISA Kit
  • Other Species Human ADH/VP/AVP ELISA KitMouse ADH ELISA KitMouse ADH/VP/AVP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ADH ELISA Kit allows for the in vitro quantitative determination of ADH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the Assay: ADH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADH antibody and an ADH-HRP conjugate. The assay sample and buffer are incubated together with ADH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH concentration since ADH from samples and ADH-HRP conjugate compete for the anti-ADH antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH from the sample, fewer sites are left to bind ADH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH concentration in each sample is interpolated from this standard curve.




tag

Inquiry About Human ADH ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!