Human DNM1 ELISA Kit

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  • Alternative name

    Human DNM ELISA Kit;Human EIEE31 ELISA Kit;Human dynamin 1 ELISA Kit;Human dynamin-1 ELISA Kit;

  • Catalog
    E012504
  • species
    Human
  • GeneDNM1
  • Other Species Mouse Dnm1 ELISA KitBovine DNM1 ELISA KitRat Dnm1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DNM1. No significant cross-reactivity or interference between DNM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNM1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DNM1 ELISA Kit allows for the in vitro quantitative determination of DNM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This BG DNM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DNM1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY DNM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DNM1 antibody and an DNM1-HRP conjugate. The assay sample and buffer are incubated together with DNM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNM1 concentration since DNM1 from samples and DNM1-HRP conjugate compete for the anti-DNM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DNM1 from the sample, fewer sites are left to bind DNM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNM1 concentration in each sample is interpolated from this standard curve.
  • Human Dynamin-1 Protein information
  • Uniprot ID DYN1_HUMAN
  • Uniprot AC Q05193; A6NLM6; Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2;
  • UniGene Hs.522413;
  • GeneID 1759
  • KEGG hsa:1759;
  • Human Dynamin-1 Protein SEQUENCE
  • SEQUENCE 864 AA; 97408 MW; 7FCD8CB572FFEAEF CRC64;

    MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN

    FVGRDFLPRG SGIVTRRPLV LQLVNATTEY AEFLHCKGKK FTDFEEVRLE

    IEAETDRVTG TNKGISPVPI NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP

    DIEFQIRDML MQFVTKENCL ILAVSPANSD LANSDALKVA KEVDPQGQRT

    IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIDGKKDITA

    ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL

    QSQLLSIEKE VEEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ

    IDTYELSGGA RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT

    GLFTPDMAFE TIVKKQVKKI REPCLKCVDM VISELISTVR QCTKKLQQYP

    RLREEMERIV TTHIREREGR TKEQVMLLID IELAYMNTNH EDFIGFANAQ

    QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE YWFVLTAENL

    SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY

    RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS

    MDPQLERQVE TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE

    LLANLYSCGD QNTLMEESAE QAQRRDEMLR MYHALKEALS IIGDINTTTV

    STPMPPPVDD SWLQVQSVPA GRRSPTSSPT PQRRAPAVPP ARPGSRGPAP

    GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP PGVPSRSGQA

    SPSRPESPRP PFDL

  • UCSC uc064wcg.1; human. [Q05193-1];


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