Human DUSP5 ELISA Kit

Alternative name
Human dual specificity protein phosphatase hVH3 ELISA Kit;Human VH3 ELISA Kit;Human DUSP ELISA Kit;Human HVH3 ELISA Kit;Human dual specificity phosphatase 5 ELISA Kit;Human dual specificity protein phosphatase 5 ELISA Kit;Human VH1-like phosphatase 3 ELISA Kit;Human serine/threonine specific protein phosphatase ELISA Kit;
Catalog
E012454
species
Human
GeneDUSP5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 5 (DUSP5). No significant cross-reactivity or interference between Dual Specificity Phosphatase 5 (DUSP5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman DUSP5 ELISA Kit allows for the in vitro quantitative determination of DUSP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 5 (DUSP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Phosphatase 5 (DUSP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dual specificity protein phosphatase 5 Protein information
Uniprot ID DUS5_HUMAN
Uniprot AC Q16690; Q12997; Q5T603;
UniGene Hs.2128;
GeneID 1847
KEGG hsa:1847;
Human Dual specificity protein phosphatase 5 Protein SEQUENCE
SEQUENCE 384 AA; 42047 MW; 2E4B2938F886CB4E CRC64;
MKVTSLDGRQ LRKMLRKEAA ARCVVLDCRP YLAFAASNVR GSLNVNLNSV
VLRRARGGAV SARYVLPDEA ARARLLQEGG GGVAAVVVLD QGSRHWQKLR
EESAARVVLT SLLACLPAGP RVYFLKGGYE TFYSEYPECC VDVKPISQEK
IESERALISQ CGKPVVNVSY RPAYDQGGPV EILPFLYLGS AYHASKCEFL
ANLHITALLN VSRRTSEACA THLHYKWIPV EDSHTADISS HFQEAIDFID
CVREKGGKVL VHCEAGISRS PTICMAYLMK TKQFRLKEAF DYIKQRRSMV
SPNFGFMGQL LQYESEILPS TPNPQPPSCQ GEAAGSSLIG HLQTLSPDMQ
GAYCTFPASV LAPVPTHSTV SELSRSPVAT ATSC
UCSC uc001kzd.4; human.;

Category

Human DUSP5 ELISA Kit