Human DUSP27 ELISA Kit

Alternative name
Human dual specificity phosphatase 27 (putative) ELISA Kit;Human inactive dual specificity phosphatase 27 ELISA Kit;
Catalog
E012449
species
Human
GeneDUSP27
SpecificityThis assay has high sensitivity and excellent specificity for detection of human DUSP27. No significant cross-reactivity or interference between human DUSP27 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human DUSP27 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman DUSP27 ELISA Kit allows for the in vitro quantitative determination of DUSP27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DUSP27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DUSP27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP27 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Inactive dual specificity phosphatase 27 Protein information
Uniprot ID DUS27_HUMAN
Uniprot AC Q5VZP5; A0AUM4; Q9C074;
UniGene Hs.632462;
GeneID 92235
KEGG hsa:92235;
Human Inactive dual specificity phosphatase 27 Protein SEQUENCE
SEQUENCE 1158 AA; 130176 MW; 6843A7062D80CBB2 CRC64;
MATRKDTEEE QVVPSEEDEA NVRAVQAHYL RSPSPSQYSM VSDAETESIF
MEPIHLSSAI AAKQIINEEL KPPGVRADAE CPGMLESAEQ LLVEDLYNRV
REKMDDTSLY NTPCVLDLQR ALVQDRQEAP WNEVDEVWPN VFIAEKSVAV
NKGRLKRLGI THILNAAHGT GVYTGPEFYT GLEIQYLGVE VDDFPEVDIS
QHFRKASEFL DEALLTYRGK VLVSSEMGIS RSAVLVVAYL MIFHNMAILE
ALMTVRKKRA IYPNEGFLKQ LRELNEKLME EREEDYGREG GSAEAEEGEG
TGSMLGARVH ALTVEEEDDS ASHLSGSSLG KATQASKPLT LIDEEEEEKL
YEQWKKGQGL LSDKVPQDGG GWRSASSGQG GEELEDEDVE RIIQEWQSRN
ERYQAEGYRR WGREEEKEEE SDAGSSVGRR RRTLSESSAW ESVSSHDIWV
LKQQLELNRP DHGRRRRADS MSSESTWDAW NERLLEIEKE ASRRYHAKSK
REEAADRSSE AGSRVREDDE DSVGSEASSF YNFCSRNKDK LTALERWKIK
RIQFGFHKKD LGAGDSSGEP GAEEAVGEKN PSDVSLTAYQ AWKLKHQKKV
GSENKEEVVE LSKGEDSALA KKRQRRLELL ERSRQTLEES QSMASWEADS
STASGSIPLS AFWSADPSVS ADGDTTSVLS TQSHRSHLSQ AASNIAGCST
SNPTTPLPNL PVGPGDTISI ASIQNWIANV VSETLAQKQN EMLLLSRSPS
VASMKAVPAA SCLGDDQVSM LSGHSSSSLG GCLLPQSQAR PSSDMQSVLS
CNTTLSSPAE SCRSKVRGTS KPIFSLFADN VDLKELGRKE KEMQMELREK
MSEYKMEKLA SDNKRSSLFK KKKVKEDEDD GVGDGDEDTD SAIGSFRYSS
RSNSQKPETD TCSSLAVCDH YASGSRVGKE MDSSINKWLS GLRTEEKPPF
QSDWSGSSRG KYTRSSLLRE TESKSSSYKF SKSQSEEQDT SSYHEANGNS
VRSTSRFSSS STREGREMHK FSRSTYNETS SSREESPEPY FFRRTPESSE
REESPEPQRP NWARSRDWED VEESSKSDFS EFGAKRKFTQ SFMRSEEEGE
KERTENREEG RFASGRRSQY RRSTDREEEE EMDDEAIIAA WRRRQEETRT
KLQKRRED
UCSC uc001geb.2; human.;

Category

Human DUSP27 ELISA Kit

Human dual specificity phosphatase 27 (putative) ELISA Kit;Human inactive dual specificity phosphatase 27 ELISA Kit;