Human MAP2K5 ELISA Kit

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  • Alternative name

    Human MAP kinase kinase 5 ELISA Kit;Human MAPKK 5 ELISA Kit;Human MAPK/ERK kinase 5 ELISA Kit;Human MEK 5 ELISA Kit;Human MEK5 ELISA Kit;Human MKK5 ELISA Kit;Human PRKMK5 ELISA Kit;Human HsT17454 ELISA Kit;Human MAPKK5 ELISA Kit;Human mitogen-activated protein kinase kinase 5 ELISA Kit;Human dual specificity mitogen-activated protein kinase kinase 5 ELISA Kit;Human MAP kinase kinase MEK5b ELISA Kit;

  • Catalog
    E012428
  • species
    Human
  • GeneMAP2K5
  • Other Species Mouse Map2k5 ELISA KitRat Map2k5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman MAP2K5 ELISA Kit allows for the in vitro quantitative determination of MAP2K5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MAP2K5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MAP2K5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAP2K5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAP2K5 can be calculated.
  • Human Dual specificity mitogen-activated protein kinase kinase 5 Protein information
  • Uniprot ID MP2K5_HUMAN
  • Uniprot AC Q13163; B4DE43; Q92961; Q92962;
  • UniGene Hs.114198;
  • GeneID 5607
  • KEGG hsa:5607;
  • Human Dual specificity mitogen-activated protein kinase kinase 5 Protein SEQUENCE
  • SEQUENCE 448 AA; 50112 MW; F23BB327E2A9C7DC CRC64;

    MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ

    VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE

    PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA

    ELKKILANGQ MNEQDIRYRD TLGHGNGGTV YKAYHVPSGK ILAVKVILLD

    ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC TEFMDGGSLD

    VYRKMPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTRGQVKLC

    DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA

    LGRFPYPQIQ KNQGSLMPLQ LLQCIVDEDS PVLPVGEFSE PFVHFITQCM

    RKQPKERPAP EELMGHPFIV QFNDGNAAVV SMWVCRALEE RRSQQGPP

  • UCSC uc002aqu.4; human. [Q13163-1];


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