Human DCLK1 ELISA Kit

Alternative name
Human doublecortin domain-containing protein 3A ELISA Kit;Human doublecortin-like and CAM kinase-like 1 ELISA Kit;Human Doublecortin-like kinase 1 ELISA Kit;Human DCAMKL1 ELISA Kit;Human DCDC3A ELISA Kit;Human KIAA0369 ELISA Kit;Human CL1 ELISA Kit;Human CLICK1 ELISA Kit;Human DCLK ELISA Kit;Human doublecortin like kinase 1 ELISA Kit;Human serine/threonine-protein kinase DCLK1 ELISA Kit;
Catalog
E012356
species
Human
GeneDCLK1
Standard Curve
Other Species Mouse Dclk1 ELISA Kit Rat Dclk1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Doublecortin Like Kinase 1 (DCLK1). No significant cross-reactivity or interference between Doublecortin Like Kinase 1 (DCLK1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman DCLK1 ELISA Kit allows for the in vitro quantitative determination of DCLK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin Like Kinase 1 (DCLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin Like Kinase 1 (DCLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin Like Kinase 1 (DCLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Doublecortin Like Kinase 1 (DCLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serine/threonine-protein kinase DCLK1 Protein information
Uniprot ID DCLK1_HUMAN
Uniprot AC O15075; B7Z3E9; Q5VZY8; Q5VZZ0; Q5VZZ1;
UniGene Hs.507755; Hs.728619;
GeneID 9201
KEGG hsa:9201;
Human Serine/threonine-protein kinase DCLK1 Protein SEQUENCE
SEQUENCE 740 AA; 82224 MW; D7B6D855099A315C CRC64;
MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL
SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN
LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW
SVNVKTTSAS RAVSSLATAK GSPSEVRENK DFIRPKLVTI IRSGVKPRKA
VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG
DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASS SRRSTTKSPG
PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRSSQHG
GSSTSLASTK VCSSMDENDG PGEEVSEEGF QIPATITERY KVGRTIGDGN
FAVVKECVER STAREYALKI IKKSKCRGKE HMIQNEVSIL RRVKHPNIVL
LIEEMDVPTE LYLVMELVKG GDLFDAITST NKYTERDASG MLYNLASAIK
YLHSLNIVHR DIKPENLLVY EHQDGSKSLK LGDFGLATIV DGPLYTVCGT
PTYVAPEIIA ETGYGLKVDI WAAGVITYIL LCGFPPFRGS GDDQEVLFDQ
ILMGQVDFPS PYWDNVSDSA KELITMMLLV DVDQRFSAVQ VLEHPWVNDD
GLPENEHQLS VAGKIKKHFN TGPKPNSTAA GVSVIATTAL DKERQVFRRR
RNQDVRSRYK AQPAPPELNS ESEDYSPSSS ETVRSPNSPF
UCSC uc001uve.4; human. [O15075-1];

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Human DCLK1 ELISA Kit